SRF在胃癌细胞中的表达及其意义

左静; 刘亮; 赵敏; 左连富

SRF在胃癌细胞中的表达及其意义

SRF expression and its biological significance in gastric carcinoma cells

摘要

摘要:
目的研究胃癌细胞中血清应答因子(serum response factor, SRF)的表达水平, 探讨SRF的表达与胃癌发生、发展关系。
方法利用RNAi技术沉默胃癌SGC-7901细胞SRF基因, 荧光显微镜检测转染效率, CCK-8法检测细胞增殖情况, Real-time PCR法检测SRF基因表达水平, Western blot法检测SRF蛋白表达水平, 流式细胞术检测细胞周期。
结果 RNAi技术沉默胃癌SGC-7901细胞SRF基因后SRF蛋白下调为空白对照组的40.1%, 有显著性差异(P < 0.05)。与空白对照组及阴性对照组相比, siRNA转染组细胞增殖明显受抑制, 抑制率为64.24%, 周期阻滞于G₀/G₁期(P < 0.05)。
结论 SRF在胃癌细胞中表达可促进胃癌细胞增殖, 以此促进胃癌的发生、发展。SRF很可能是胃癌防治的一个重要靶点。

Abstract:
Objective This study aims to explore the relationship between serum response factor (SRF) expression level and gastric cancer progression by detecting SRF expression level in cancer cells.
Methods The SRF gene in SGC-7901 cells was silenced by RNA interference.Transfection efficiency was detected by fluorescence microscopy, cell proliferation by CCK 8 method, SRF gene and protein expression level by real-time polymerase chain reaction and Western blot, and cell cycle by flow cytometry.
Results Cell treatment with siRNA-SRF induced significant reduction in SRF mRNA levels.Western blot analysis showed that SRF protein decreased by 40.1% in the siRNA group compared with that in the control group (P < 0.05).Compared with the blank, negative, and mock transfection control groups, cell proliferation of the siRNA-SRF group decreased.The inhibition ratio reached 64.24%, as measured by the CCK-8 assay (P < 0.05).Treatment with siRNA could block SGC-7901 cell cycle at G₀/G₁ phase (P < 0.05).
Conclusion SRF expression is closely associated with gastric carcinoma cell proliferation.SRF protein level detection can provide a certain reference value in evaluating malignant gastric carcinoma progression.SRF is possibly an important target for the prevention and control of gastric cancers.

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