Abstract:
Objective This study aims to compare the difference in the expression and localization of brain-specific angiogenesis inhibitor 1(bai-1) between human normal and clear cell carcinoma of kidney tissues at different clinical stages.this study also aims to explore the mechanism of bai-1-mediated inhibition of tumor endothelial cell proliferation.
Methods A total of 133 human normal and cancerous kidney tissues were obtained.the tissue localized more than 4 cm away from the cancerous kidney tissue was identified as the normal control kidney tissue.immunohistochemical staining was conducted to detect the expression of bai-1, vegf, mvd, and p53.the expression was detected with the respective antibodies for quantitative statistical analysis.fresh renal cell carcinoma tissue samples were obtained from 27 patients, and 15 tissue samples were obtained 4 cm away from the cancer.the expression of bai-1 in the renal cell carcinoma tissue was examined through western blot analysis.
Results The bai-1 expression level in the normal kidney tissues is much higher compared with that in the kidney cancer tissues, which ranged from low and moderate to high differentiated stages.statistical analysis results revealed a correlation between the expression of bai-1 and vegf, mvd, and p53 proteins.
Conclusion The expression level of bai-1 in kidney cancer tissues is very low, even difficult to detect, compared with the increased expression of vegf and cd34.this finding suggests that bai-1 can inhibit tumor angiogenesis.the level of p53 is positively correlated with the expression of bai-1 according to the data derived with the spearman software, suggesting the existence of crosstalk between these two molecules.
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