张默涵, 葛振宇, 谭杨, 谷金华, 史立宏, 张小茜. 肠道菌群与结直肠癌进展关系的临床研究探讨[J]. 中国肿瘤临床, 2022, 49(6): 293-298. DOI: 10.12354/j.issn.1000-8179.2022.20211184
引用本文: 张默涵, 葛振宇, 谭杨, 谷金华, 史立宏, 张小茜. 肠道菌群与结直肠癌进展关系的临床研究探讨[J]. 中国肿瘤临床, 2022, 49(6): 293-298. DOI: 10.12354/j.issn.1000-8179.2022.20211184
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Mohan Zhang, Zhenyu Ge, Yang Tan, Jinhua Gu, Lihong Shi, Xiaoqian Zhang. Clinical study on the relationship between gut microbiota and the progression of colorectal cancer[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2022, 49(6): 293-298. DOI: 10.12354/j.issn.1000-8179.2022.20211184
Citation: Mohan Zhang, Zhenyu Ge, Yang Tan, Jinhua Gu, Lihong Shi, Xiaoqian Zhang. Clinical study on the relationship between gut microbiota and the progression of colorectal cancer[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2022, 49(6): 293-298. DOI: 10.12354/j.issn.1000-8179.2022.20211184
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肠道菌群与结直肠癌进展关系的临床研究探讨

Clinical study on the relationship between gut microbiota and the progression of colorectal cancer

    摘要
  • 摘要:
      目的  探讨结直肠癌不同进展过程中患者肠道的优势菌群以及对结直肠癌进展的影响。
      方法  回顾性分析 2020年1月至2021年5月于潍坊医学院附属医院就诊的20例结直肠癌患者、20例腺瘤性息肉患者和20例健康受试者，分别设为C组、A组和N组，分别收集粪便以及肿瘤组织、息肉组织及肠壁组织。利用16sRNA技术对收集的粪便进行高通量测序，比较微生物的丰度与多样性，并利用免疫组织化学法检测肿瘤组织、息肉组织以及肠壁组织白细胞介素-6（interleukin 6，IL-6）的表达水平。
      结果  稀释性曲线显示所有样本包含的OTU数目不同；采用α多样性、β多样性、PCoA等分析显示：不同样本间及组间菌群的丰度及多样性有显著性差异。LEfSe分析显示差异最显著的菌为C组Prevotellaceae、A组Enterobacteriaceae、Erysipelotrichaceae和N组Ruminococcaceae、Bifidobacteriaceae。免疫组织化学结果显示IL-6在C组中的阳性率高于A组和N组。
      结论  在C组、A组与N组中，每个样本的菌群的丰度与多样性并不相同，各组具有明显不同的群落结构，呈现出组内样本差异较小，组间样本差异较大的现象（P<0.05）。结直肠癌可能与Prevotellaceae有关，而腺瘤性息肉可能与Enterobacteriaceae、Erysipelotrichaceae有关。慢性炎症可能通过产生IL-6等炎性因子导致癌前病变，进而进展为结直肠癌。

     

    Abstract:
      Objective  To investigate the dominant intestinal flora in patients at different stages of colorectal cancer and its effect on the progression of colorectal cancer.
      Methods  Patients with colorectal cancer (group C), with adenomatous polyps (group A), and healthy subjects (group N) treated in Affiliated Hospital of Weifang Medical University from January 2020 to May 2021 were recruited; 20 of each were assigned into groups C, A and N, respectively. Their feces were collected, as well as tumor, polyp and intestinal wall tissues from each group, respectively. The feces collected from each group were sequenced by 16s RNA technology, and the abundance and diversity of fecal microorganisms in the three groups were compared; the expression levels of IL-6 in tumor tissues of group C, polyp tissues of group A and intestinal wall tissues of group N were detected by immunohistochemistry.
      Results  The rarefaction curve shows that all samples contain different numbers of OTU. The analysis of α diversity, β diversity, and PCoA showed that there were great differences in the abundance and diversity of microflora among different samples and groups. LEfSe analysis showed that the bacteria with the most significant differences were Prevotellaceae in group C, Enterobacteriaceae and Erysipelotrichaceae in group A, and Ruminococcaceae and Bifidobacteriaceae in group N. The results of immunohistochemistry showed that the positive rate of IL-6 in group C was higher than that in group A and N.
      Conclusions  For all three groups, the abundance and diversity of intestinal flora in each sample are different. Each group has significantly different community structures showing the phenomenon of small sample difference within the group and large sample difference between the groups; the difference between the groups is statistically significant (P<0.05). Colorectal cancer may be related to Prevotellaceae, while adenomatous polyps may be related to Enterobacteriaceae and Erysipelotrichaceae. The progression of colorectal cancer may be related to IL-6.

     

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