Abstract: Neuroblastoma (NB) is a pediatric malignant tumor characterized by high heterogeneity, and the stemness of cancer cells is one of the determining factors for intra-tumor heterogeneity. The stemness of NB cells plays an important role in its occurrence, development, treatment resistance, recurrence, and metastasis. Type-I cell is known as the stemness subgroup of NB, and many possible markers of this subgroup have been proposed in the recent years. The stemness of NB is regulated by several factors, such as the tumor microenvironment. Many non-coding RNAs and signaling pathways also play important roles in the regulation of stemness in NB cells. This review summarizes the latest research progress on cancer cell stemness in NB.