Abstract:
Objective To construct a prognostic model of disulfidptosis-related lncRNAs (DRLncs) to predict the prognosis of osteosarcoma and improve the survival rate of patients.
Methods Differential expression and Pearson correlation analysis were used to identify osteosarcoma-associated DRLncs. The DRLncs risk prognosis model was constructed by univariate Cox, least absolute shrinkage and selection operator (LASSO) regression analyses. The reliability of the model was verified by survival analysis, receiver operating characteristic (ROC) curves, nomograms, and calibration curves. The relationships between prognostic models, the immune microenvironment, and drug sensitivity were explored. Select 30 cases of tumor tissue from osteosarcoma patients and 30 cases of normal tissue from healthy individuals from The First Affiliated Hospital of Guangxi University of Chinese Medicine in the past 10 years, real-time quantitative PCR (qRT-PCR) was used to verify the expression of DRLncs in osteosarcoma tissues.
Results A risk prognostic model was successfully constructed with three DRLncs (RERG−IT1, AL035446.1, and AC010894.1). The model showed good performance in predicting the overall survival of osteosarcoma patients. There was a significant correlation between the DRLncs prognostic model and the tumor microenvironment, immune infiltrating cells, and chemotherapy drug sensitivity. qRT-PCR revealed significantly increased expression of RERG−IT1 in osteosarcoma tissues, while AL035446.1 and AC010894.1 were significantly reduced (all P<0.01).
Conclusions The DRLncs prognostic model constructed in this study can accurately predict the prognosis of osteosarcoma patients, which provides a new idea for the personalized treatment of osteosarcoma.