刘笑, 郗彦凤, 步鹏, 赵国海, 靳佩玉, 冯玉婷, 崔巍, 徐菁. ITGA3表达与结直肠癌免疫细胞浸润的关系[J]. 中国肿瘤临床, 2023, 50(23): 1196-1202. DOI: 10.12354/j.issn.1000-8179.2023.20231043
引用本文: 刘笑, 郗彦凤, 步鹏, 赵国海, 靳佩玉, 冯玉婷, 崔巍, 徐菁. ITGA3表达与结直肠癌免疫细胞浸润的关系[J]. 中国肿瘤临床, 2023, 50(23): 1196-1202. DOI: 10.12354/j.issn.1000-8179.2023.20231043
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Xiao Liu, Yanfeng Xi, Peng Bu, Guohai Zhao, Peiyu Jin, Yuting Feng, Wei Cui, Jing Xu. Relationship between ITGA3 expression and immune cell infiltration in colorectal cancer[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2023, 50(23): 1196-1202. DOI: 10.12354/j.issn.1000-8179.2023.20231043
Citation: Xiao Liu, Yanfeng Xi, Peng Bu, Guohai Zhao, Peiyu Jin, Yuting Feng, Wei Cui, Jing Xu. Relationship between ITGA3 expression and immune cell infiltration in colorectal cancer[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2023, 50(23): 1196-1202. DOI: 10.12354/j.issn.1000-8179.2023.20231043
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ITGA3表达与结直肠癌免疫细胞浸润的关系

Relationship between ITGA3 expression and immune cell infiltration in colorectal cancer

    摘要
  • 摘要:
      目的  探讨结直肠癌（colorectal cancer，CRC）中整合素亚蛋白ɑ3（integrin subunit ɑ3，ITGA3）的表达与免疫细胞浸润之间的关系。
      方法  利用生物信息学方法分析ITGA3 mRNA在泛癌及CRC组织中的表达及对预后的影响，同时全面研究ITGA3与肿瘤浸润免疫细胞的相关性。收集2021年1月至2021年12月山西省肿瘤医院确诊为CRC的病例233例，进一步采用免疫组织化学染色（IHC）检测ITGA3、CD8、CD163、FOXP3、PD-L1、CTLA-4、PD-1在CRC组织中的表达，分析ITGA3与免疫浸润细胞、免疫检查点之间的关系。
      结果  生信分析结果显示ITGA3 mRNA在CRC中表达升高；ITGA3高表达与无进展生存期（progression-free survival，PFS）相关（P<0.05）；单因素、多因素分析显示年龄、分期与患者的预后显著相关（P<0.05）。此外，上调的ITGA3表达与CRC中多种免疫细胞浸润水平密切相关。IHC结果表明ITGA3在CRC组织中的高表达率明显高于癌旁正常组织（P<0.05）。ITGA3的表达与淋巴结转移相关（P<0.05），与CD8+T细胞、PD-L1、CTLA-4（均P<0.05）等免疫标志物表达相关。
      结论  ITGA3在CRC中高表达，与免疫细胞浸润等密切相关，其可能调节肿瘤免疫微环境，为临床治疗及预后判断提供了新的思路。

     

    Abstract:
      Objective  To explore the relationship between integrin ɑ3 (ITGA3) expression and immune cell infiltration in colorectal cancer (CRC).
      Methods  Bioinformatic methods were used to analyze ITGA3 mRNA expression in pan-cancer and CRC tissues, as well as its association with CRC prognosis. The correlation between ITGA3 and tumor-infiltrating immune cells was also investigated. In total, 233 cases of CRC diagnosed at Shanxi Provincial Cancer Hospital between January and December 2021 were included, and ITGA3, CD8, CD163, FOXP3, PD-L1, CTLA-4, and PD-1 expression in CRC tissues were determined by immunohistochemistry (IHC) to analyze the relationship between ITGA3 and infiltrating immune cells and immune checkpoints.
      Results  Bioinformatics analysis showed elevated ITGA3 mRNA levels in CRC. High ITGA3 expression was associated with PFS (P<0.05). Univariate and multifactorial analyses showed that age and stage were significantly correlated with prognosis (P<0.05). In addition, ITGA3 upregulation was closely correlated with multiple immune cell infiltration levels in CRC. Furthermore, IHC results showed that ITGA3 expression in CRC tissues was significantly higher than that in adjacent normal tissues (P<0.05). ITGA3 expression was associated with lymph node metastasis (P<0.05) and correlated with the expression of immune markers, such as CD8+T-cells, PD-L1, and CTLA-4 (P<0.05).
      Conclusions  ITGA3 is highly expressed in CRC, which is closely related to immune cell infiltration and may regulate the tumor immune microenvironment, which provides a new idea for clinical treatment and a potential new independent predictive marker.

     

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