Abstract: Objective:To investigate the expression of Bmi- 1 in normal gastric mucosa, gastric carcinoma and precancerous lesions; to analyze the correlation of Bmi- 1, ki- 67and Bcl- 2 with the distribution of Bmi-1(+) cells and Bcl-2 (+) /ki- 67(-) cells; and to explore the correlation between Bmi- 1 expression with clinicopatho -logic features of gastric carcinoma. Methods:Envision immunohistochemistry and immunohistochemical dou-ble-labelling staining were used to detect the expression of Bmi- 1, ki- 67, Bcl- 2 and the distribution of Bcl- 2 (+) /ki- 67(-) cells in 162 gastric carcinoma specimens. Results: The expression of Bmi- 1 in gastric carcinoma specimens (52.5%) was significantly higher than in normal gastric mucosa (21.6%) ( P<0.05). The expression of Bmi-1 was related to Lauren's classification, Borrmann classification and clinical pathological stage (P<0.05). The expression of ki- 67was related to Borrmann classification in gastric carcinoma (P<0.05). The ex-pression of Bcl-2 was related to Lauren's classification (P<0.05). In gastric carcinoma and intestinal metapla-sia tissues, Bmi-1 expression was positively correlated with Bcl-2 (P<0.05). Conclusion:The variation of Bmi-1 expression in gastric carcinoma and precancerous lesions might be involved in cell proliferation, apoptosis and cancerization. In gastric cancer and intestinal metaplasia, Bmi-1 is significantly associated with progres -sion of gastric carcinoma.