薛丽英, 张祥宏, 李学民①, 李月红, 丁 涛, 米建民②, 王俊灵, 严 霞, 温 实, 邢 欣, 崔晋峰. 食管鳞状细胞癌CD40COX-2 表达及其与血管生成关系的研究*[J]. 中国肿瘤临床, 2010, 37(1): 36-39. DOI: 10.3969/j.issn.1000-8179.2010.01.010
引用本文: 薛丽英, 张祥宏, 李学民①, 李月红, 丁 涛, 米建民②, 王俊灵, 严 霞, 温 实, 邢 欣, 崔晋峰. 食管鳞状细胞癌CD40COX-2 表达及其与血管生成关系的研究*[J]. 中国肿瘤临床, 2010, 37(1): 36-39. DOI: 10.3969/j.issn.1000-8179.2010.01.010
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XUE Liying1, ZHANG Xianghong1, LI Xuemin2, LI Yuehong1, DING Tao3, MI Jianmin4, WANG Junling3, YANXia3, WEN Shi3, XING Xin3, CUI Jinfeng3. Expression of CD40 and COX- 2 and Its Relationship with Angiogenesis in Esophageal Squamous Cell Carcinoma[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2010, 37(1): 36-39. DOI: 10.3969/j.issn.1000-8179.2010.01.010
Citation: XUE Liying1, ZHANG Xianghong1, LI Xuemin2, LI Yuehong1, DING Tao3, MI Jianmin4, WANG Junling3, YANXia3, WEN Shi3, XING Xin3, CUI Jinfeng3. Expression of CD40 and COX- 2 and Its Relationship with Angiogenesis in Esophageal Squamous Cell Carcinoma[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2010, 37(1): 36-39. DOI: 10.3969/j.issn.1000-8179.2010.01.010
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食管鳞状细胞癌CD40COX-2 表达及其与血管生成关系的研究*

Expression of CD40 and COX- 2 and Its Relationship with Angiogenesis in Esophageal Squamous Cell Carcinoma

    摘要
  • 摘要: 目的：探讨CD40和COX-2 在食管鳞状细胞癌组织中的表达情况、临床病理意义及其与肿瘤血管生成的关系。方法：采用免疫组织化学方法研究79例食管癌和28例正常食管黏膜上皮组织中CD40和COX-2 的表达情况，根据CD34表达计算食管癌组织平均微血管密度（MVD）；分析CD40、COX-2 的表达与食管癌发生部位、肿瘤大小、分化程度、MVD及淋巴结转移的关系。同时采用免疫细胞化学染色及Western Blot方法检测食管癌Eca109 细胞和原代培养的正常食管上皮细胞CD40和COX-2 在蛋白水平上表达的差异。结果：免疫组化结果表明，食管癌组织中CD40和COX-2 的阳性表达率均明显高于正常食管鳞状上皮（54.43% vs 10.71% ，69.62% vs 17.86% ，P 均<0.05）。CD40在食管癌中的表达与淋巴结转移有关，伴有淋巴结转移者CD40阳性表达率明显高于无淋巴结转移者（70.37% vs 46.15% ，P<0.05），但CD40表达与其他临床病理特征无明显相关。COX-2 在食管癌中的阳性表达与肿瘤大小、部位、分化程度、有无淋巴结转移等均无明显相关（P>0.05）。 CD40与COX-2 在食管癌组织中表达明显相关（P<0.05），相关系数（Φ）为0.446。食管癌组织中MVD明显高于正常食管组织（25.02± 5.52vs 12.09± 4.55，P<0.05）。 CD40和COX-2 阳性表达组食管癌组织MVD明显高于阴性表达组（26.37± 6.02vs 22.58± 5.25，P<0.05）。 体外研究结果表明，食管癌Eca109 细胞CD40和COX-2 表达均高于正常食管上皮细胞（P<0.05）。 结论：CD40表达与食管鳞状细胞癌的发生、发展密切相关，CD40可能通过影响COX-2 的表达促进食管癌组织微血管生成。

     

    Abstract: Objective: To explore the putative role of CD40and COX-2 expression in human esophageal squamous cell carcinoma (ESCC) and to analyze their possible relationship with angiogenesis in ESCC.Methods: The expression of CD 40and COX-2 was detected in79ESCC and 28normal esophageal epithelial tis-sue samples with immunohistochemical staining. The microvessel density by CD34was determined and the clinicopathological significance of CD 40and COX- 2 expression in ESCC was analyzed. In addition, the ex -pression of CD 40and COX-2 in esophageal squamous cell carcinoma cell line Eca 109 and primary cultured normal esophageal epithelial cells in vivo was comparatively studied with immunocytochemical staining and Western blot. Results: Compared with that in normal epithelial tissues, the expression of CD40and COX-2 in ESCC was significantly higher (54.43% vs10.71%;69.62% vs17.86%; respectively, P<0.05). The positive expression rate of CD 40in ESCC cases with lymph node metastasis was significantly higher than that in those without lymph node metastasis (70.37% vs46.15%,P<0.05). No correlation was found between CD 40expres-sion and patient age, sex, tumor location, size and differentiation of tumors. No clinicopathological signifi -cance of COX- 2 expression in ESCC was found. There was a positive correlation between CD 40expression and COX-2 expression in esophageal squamous cell carcinoma ( P<0.05, Φ =0.446 ). The mean MVD value in ESCC was significantly higher than that in normal esophageal tissue (25.02± 5.52vs12.09± 4.55, P<0.05). MVD value in ESCC was closely correlated with lymph node metastasis. The mean MVD value in ESCC cas-es with positive CD 40and COX-2 expression was higher than that in those with negative CD 40and COX-2 expression (26.37± 6.02vs22.58± 5.25, P<0.05). Western blot results showed that CD 40and COX-2 expres-sion in Eca109 was higher than that in cultured normal esophageal epithelial cells (P<0.05). Conclusion:The expression of CD 40is involved in the carcinogenesis and progression of esophageal squamous cell carcino -ma. CD40may play an important role in the angiogenesis of ESCC through promoting COX-2 expression.

     

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