Abstract:
Objective:To investigate the influence of Mannan Peptide combined with 5-Fu on the prolifera -tion and apoptosis of HepG 2 cells and to explore the related mechanism. Methods:HepG2 cells were divided into four groups: the control group, 5-Fu group, Mannan Peptide group and combination group. Cell proliferation was evaluated by MTT assay. Flow cytometry was used to examine the cell cycle, proliferation index (PI) and apoptosis ratio. The expression of Bcl- 2 protein in each group was analyzed by immunocytochemical method. Results: The inhibitive effect of 5-Fu plus Mannan Peptide was significantly stronger than that of Man-nan Peptide alone, in a time- and dose-dependent manner. Cells were treated with medicine for 48hours. Compared with cells in the control group and 5-Fu group, the percentage of cells in G0/G1 stage and the apop -tosis ratio in the combination group were increased ( P<0.01). In immunocytochemical analysis, the combina -tion of Mannan Peptide and 5-Fu could significantly decrease Bcl- 2 protein level (P<0.01). Conclusion:The combination of Mannan Peptide and 5-Fu can synergistically suppress the proliferation and induce apoptosis of human hepatoma HepG 2 cells. The mechanism might be associated with down-regulation of Bcl-2 protein expression.