赵 华①, 李 慧, 于津浦, 苏延军②, 杨莉莉, 魏 枫, 安秀梅, 徐 峰②, 岳东升②, 赵晓亮②, 任秀宝. TSLP在肺癌组织中的表达及其与调节性T 细胞的相关性*[J]. 中国肿瘤临床, 2010, 37(3): 126-130. DOI: 10.3969/j.issn.1000-8179.2010.03.002
引用本文: 赵 华①, 李 慧, 于津浦, 苏延军②, 杨莉莉, 魏 枫, 安秀梅, 徐 峰②, 岳东升②, 赵晓亮②, 任秀宝. TSLP在肺癌组织中的表达及其与调节性T 细胞的相关性*[J]. 中国肿瘤临床, 2010, 37(3): 126-130. DOI: 10.3969/j.issn.1000-8179.2010.03.002
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ZHAO Hua1, 3, 4, LI Hui1, 3, 4, YU Jinpu1, 3, 4, SU Yanjun2, 3. The Expression of TSLP and Its Relationship with the Number of Infiltrating Regulatory T Cells in Lung Cancer[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2010, 37(3): 126-130. DOI: 10.3969/j.issn.1000-8179.2010.03.002
Citation: ZHAO Hua1, 3, 4, LI Hui1, 3, 4, YU Jinpu1, 3, 4, SU Yanjun2, 3. The Expression of TSLP and Its Relationship with the Number of Infiltrating Regulatory T Cells in Lung Cancer[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2010, 37(3): 126-130. DOI: 10.3969/j.issn.1000-8179.2010.03.002
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TSLP在肺癌组织中的表达及其与调节性T 细胞的相关性*

The Expression of TSLP and Its Relationship with the Number of Infiltrating Regulatory T Cells in Lung Cancer

    摘要
  • 摘要: 目的：探讨胸腺基质淋巴生成素（thymic stromal lymphopoietin ，TSLP）在肺癌组织中的表达及其与临床指标以及局部调节性T 细胞（Regulatory T cells，Tregs）数量的相关性。方法：分别采用Q-RT-PCR 和免疫组织化学染色方法检测TSLP在不同病变类型的肺组织中的表达，分析TSLP在不同病变肺组织中的表达差异；并运用免疫组化方法检测TSLP蛋白在不同病理类型的肺癌组织中的表达，分析TSLP表达与临床病理特征之间的相关性；采用免疫组化法检测肺癌组织中的Tregs细胞，分析TSLP表达与肿瘤局部Tregs细胞数量之间的关系。结果：TSLP基因在肺癌组织、癌旁组织、非肿瘤肺上皮均表达阳性，且表达差异无统计学意义；TSLP蛋白表达于胞浆中，其在肺癌组织中的阳性表达率（69.57%）显著高于肺的良性病变（13.33%）和非肿瘤肺上皮（30.00%），且TSLP表达与肿瘤大小和淋巴结转移有关；TSLP表达阳性的患者其肿瘤局部浸润的调节性T 细胞数量明显高于TSLP阴性组（P<0.05）。 结论：TSLP蛋白在肺癌组织中表达增加，且与肿瘤局部调节性T 细胞数量增多有关，这提示TSLP可能是通过诱导Tregs增加在肺癌免疫耐受中发挥作用。

     

    Abstract: Objective:To investigate the expression of TSLP in human lung cancer tissue and the correla -tion between TSLP expression and number of regulatory T cells(Tregs). Methods:The expression of TSLP mRNA and protein was detected in different pathological lesions of the lung by Q-RT-PCR and immunohisto-chemistry. Immunohistochemistry was used to detect Foxp3 + Tregs. The correlation of TSLP with the number of Tregs was analyzed. Results: TSLP gene was expressed in tumor tissues (n=37), latero-tumor tissues ( n=29) and non-tumor lung tissues ( n=24), without statistical difference (P=0.148 ). TSLP protein was expressed in the cytoplasm and was observeed in 69.57% of tumor tissues, 13.33% of benign lesions and30.00% of non-tumor lung tissues, with a significant difference ( P<0.05). The expression of TSLP protein was correlated with tumor size ( P=0.000 ) and lymph node metastasis ( P=0.018 ). The number of Tregs in TSLP positive group was more than that in TSLP negative group (P<0.05). Conclusion:The expression of TSLP in lung tu-mor tissues is increased and is correlated with the number of Tregs, indicating that TSLP could induce Treg to play an important role in tumor immunotolerance.

     

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