P16表达缺失与胃肠道间质瘤生物学行为及预后关系*

赵占考; 姜忠敏; 何 滔; 贾文娟; 孙保存①

doi:10.3969/j.issn.1000-8179.2010.05.005

P16表达缺失与胃肠道间质瘤生物学行为及预后关系*

The Relationship of Loss of P16 Expression with Biological Behaviors and Prognosis of Gastrointestinal Stromal Tumors

摘要

摘要: 目的：探讨P16mRNA 和蛋白表达缺失与胃肠道间质瘤生物学行为及预后的关系。方法：采用反转录- 聚合酶链式反应（RT-PCR）、免疫组织化学及Western Blot法分别检测胃肠道间质瘤组织中P16mRNA 和蛋白表达情况，并对患者进行随访，分析P16表达水平与胃肠道间质瘤细胞核增殖指数、肿瘤细胞的侵袭危险性及胃肠道间质瘤患者生存预后间的关系，采用SPSS13.0统计软件包进行统计学分析。结果：胃肠道间质瘤中P16mRNA 和蛋白的阳性表达率分别为53.8%（21/39，冰冻组织）、51.3%（20/39，冰冻组织）和51.4%（37/72，石蜡组织），不同侵袭危险性间比较差异有统计学显著性（P<0.05），随着侵袭危险性的增高，其表达率呈下降趋势；且与核增殖指数Ki-67表达及患者生存期呈负相关（P<0.05）。结论：P16表达缺失与胃肠道间质瘤的浸润进展有关；P16mRNA 和蛋白的检测有助于判断胃肠道间质瘤的生物学行为和预后。

Abstract: Objective:To investigate the relationship of loss of P 16expression with biological behaviors and prognosis of gastrointestinal stromal tumors (GISTs). Methods:The expression of P16protein and mRNA was detected in GISTs tis-sues by immunohistochemistry, Western blot and RT-PCR. The prognosis was evaluated through follow up. Results:The ex-pression rates of P16protein and mRNA in GISTs were 53.8% (21/39, Frozen tissue), 51.3% (20/39, Frozen tissues) and 51.4% (37/72, Paraffin-embedded tissues), respectively. The expression of P 16was significantly different among GISTs of different aggressive risk (P<0.05). With the incease of aggressive risk, the expression of P16was deceased. The expres-sion of P 16was negtively correlated with Ki-67and patient survival (P<0.05). Conclusion:The loss of P 16expression has a positive correlation with the infiltration and progression of GIST. Detection of P 16 protein and mRNA is helpful for the evalu-taion of biological behaviors and prognosis of gastrointestinal stromal tumors.

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