Abstract: Objective: To explore the sensitivity of Kit or PDGFRA mutants related to gastrointestinal stromal tumor (GIST) to Gleevec. Methods:The recombinant plasmids of KIT Del 559 -560 , KIT Ins IPYD 579 , PDGFRA D 842 V and PDG-FRA L 839 P gene mutants were transiently transformed into the CHO cells by liposome methods. Western blot was used to detect the expression of the related protein and their phosphorylated forms after the cells were incubated with Gleevec for 90min. At72hours after incubation with Gleevec, MTT was used to detect cell proliferation. Results: Western blot results showed that Gleevec at 0.1 μ M can notably reduce phosphorylation of KIT Del559 -560 . Gleevec at 1 μ M completely blocked phosphorylation of KIT Ins IPYD579 and PDGFRA L839 P, but did not affect PDGFRA D842 V phosphorylation. MTT analy-sis indicated that growth of CHOPDGFRA L839 P was inhibited by Gleevec at1 μ M, however, CHOPDGFRA D842 V was re -sistant to Gleevec at 5 μ M. Conclusion:Gleevec can decrease the expression of phosphorylated protein CHOPDGFRA  L839 P and CHOKIT Ins IPYD 579 , and can remarkably inhibit the proliferation of cells containing PDGFRA L 839 P mutant.