刘强和, 黄 鑫, 向 秋①, 伍靖武②, 雷 迅. EGCG通过上调MnSOD 表达诱导鼻咽癌细胞株CNE-2 凋亡*[J]. 中国肿瘤临床, 2010, 37(11): 605-607. DOI: 10.3969/j.issn.1000-8179.2010.11.002
引用本文: 刘强和, 黄 鑫, 向 秋①, 伍靖武②, 雷 迅. EGCG通过上调MnSOD 表达诱导鼻咽癌细胞株CNE-2 凋亡*[J]. 中国肿瘤临床, 2010, 37(11): 605-607. DOI: 10.3969/j.issn.1000-8179.2010.11.002
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LIU Qiang-he1, HUANG Xin1, XIANG Qiu2, WU Jing-wu3, LEI Xun1. EGCG Induction of Nasopharyngeal Carcinoma Cell CNE-2 Apoptosis via Regulating Expression of MnSOD[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2010, 37(11): 605-607. DOI: 10.3969/j.issn.1000-8179.2010.11.002
Citation: LIU Qiang-he1, HUANG Xin1, XIANG Qiu2, WU Jing-wu3, LEI Xun1. EGCG Induction of Nasopharyngeal Carcinoma Cell CNE-2 Apoptosis via Regulating Expression of MnSOD[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2010, 37(11): 605-607. DOI: 10.3969/j.issn.1000-8179.2010.11.002
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EGCG通过上调MnSOD 表达诱导鼻咽癌细胞株CNE-2 凋亡*

EGCG Induction of Nasopharyngeal Carcinoma Cell CNE-2 Apoptosis via Regulating Expression of MnSOD

    摘要
  • 摘要: 目的：探讨表没食子儿茶素没食子酸酯（（-）-epigallocatechin-3-gallate，EGCG）对鼻咽癌细胞株CNE-2 的凋亡诱导作用、以及这个过程中MnSOD的表达变化特点。方法：应用噻唑蓝（MTT）法计算不同浓度EGCG 作用于CNE-2 鼻咽癌细胞后不同时间点的生长抑制率，并通过细胞荧光显微镜观察其凋亡情况，同时应用RT-PCR 法检测这个过程中MnSOD mRNA的表达变化。结果：EGCG 可以诱导CNE-2 细胞凋亡、抑制其生长，而且存在时间和剂量依赖关系，同时伴有MnSOD mRNA的表达上调。结论：EGCG 能通过上调MnSOD mRNA表达诱导鼻咽癌细胞株CNE-2 凋亡。

     

    Abstract: Objective: To discuss the induction of (-)-epigallocatechin-3-gallate (EGCG) on the apoptosis of nasopharyn -geal carcinoma (NPC) cell lines CNE-2, and the characteristics of the changes in MnSOD expression during the course. Methods:In vitro cultured CNE- 2 cells were divided into the control group and another 3 experiment groups. No interven-tions (interfering reagent) such as EGCG were added to the control group, while the 3 experimental groups, received a EGCG concentrations in the culture solution of 10, 50and 100 µg EGCG per ml. MTT (Thiazolyl blue) method was used to calculate the growth suppression ratio of CNE- 2 cell line treated by EGCG of various concentrations at different time points, with morphological observation of the apoptosis made by fluorescence microscope. At the same time, the change in the ex-pression of MnSOD mRNA level was measured using RT-PCR determination. Results: With increase of the EGCG drug concentration and extension of the culture time, the growth inhibition ratio of CNE-2 cells significantly rose up, that is, the survival rate of CNE-2 cells significantly decreased. Observation by a fluorescence microscope showed that the completely green and fluorescent tumor cells with normal growth could be seen in the control group. After the CNE-2 cells interacted with EGCG, the apoptotic reddish-yellow tumor cells could be seen by the fluorescence microscope. The expressions of MnSOD gene (around 700 bp) and internal -reference β-actin gene (around 500 bp) could be detected in the control and in the experimental groups. Compared with the control, however, the expression of MnSOD mRNA level notably increased (P < 0.05), after the CNE- 2 cells respectively interacted with the 10, 50and 100 µg/mL EGCG for 24h, 48h and 72h. Also, there was an up-regulation of the MnSOD mRNA expression with an increase of EGCG concentration and extension of the culture time. Conclusion:EGCG can induce the apoptosis of nasopharyngeal carcinoma cell line CNE-2 by means of up-regulating the expression of MnSOD mRNA.

     

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