Abstract: Objective: To investigate the clinical significance of serum squamous cell carcinoma antigen (SCC-Ag) in lymphatic metastasis of early-stage cervical cancer. Methods:Preoperative serum SCC-Ag detection was conducted in110 patients with stage IB-IIA cervical squamous cell cancer (SCC) who were admitted to the Tianjin Central Hospital of Gyne-cology and Obstetrics during a period from May 2004to December 2008, and a retrospective study of the cases in combi  nation with the related clinico- pathologic data was carried out. The correlation between the clinico-pathologic findings and SCC-Ag outcomes was statistically analyzed using Mann-Whitney U test and multiple logistic regression analysis. The re-ceptor performance curve (ROC) of the cases was drawn to decide and evaluate the threshold value of SCC-Ag in lymph node metastasis. Results: Univariate analysis showed that SCC-Ag titre correlates significantly with the FIGO staging, tu-mor size, depth of muscular infiltration and lymphatic metastasis, but not with the degree of differentiation and intravascular tumor embolus. The logistic regression analysis further confirmed that the lymph node metastasis and tumor diameter are the independent risk factors affecting serum marker levels (SCC-Ag ≥4 ng/ml). Moreover, lymphatic metastasis has more effect on the SCC-Ag. The ROC showed that the best cut off value of SCC-Ag for screening of the lymph node metastasis is 4 ng/ml. Conclusion:Preoperative detection of the serum SCC-Ag is definitely valuable in evaluating the lymphatic me -tastasis in early squamous carcinoma of the cervix, with a critical value of 4 ng/ml.