Abstract: Objective: To explore the expression of CXCR4 gene in the cholangiocarcinoma cell line QBC 939 and the biological behavior changes of the cells after treatment with lipopolysaccharide. Methods:RT-PCR and Western-blot meth-ods were performed to detect the expression of CXCR4 mRNA and CXCR4 protein in QBC 939 cells, before or after being treated with LPS, respectively. The inhibition effect of tumor cell growth and clone formation after LPS administration in QBC939 cells were detected by the MTT and flat clone formation experiment. The infestation test was used to detect infes-tation ability in QBC939 cells after treatment with LPS. Results: CXCR 4 mRNA and protein were positively expressed in cholangiocarcinoma cell line QBC939 . CXCR 4 mRNA and protein in QBC 939 cells expressed differently when exposed in different LPS concentration gradients. MTT results showed the different effects of cell growth after treatment with LPS. Cells were not affected at 24or 48hour time points when LPS was at or below 0.1 g/mL. However, when LPS concentration reached up to 1.0 g/mL or 5.0 g/mL, there were definite depressive effects in the cells. After 72hours, even with LPS concentration as low as 0.1 g/mL, the depressive effect remained. In the flat clone formation test, tumor cell clones formed in 10days in the control group, while the test groups took about 12days to form. CXCL12(SDF- 1) can induce QBC939 cells to migrate. The migration rate in the control group was ( 53.723 %±1.812 ), while the migration rates of the test groups were lower (P<0.05). Conclusion:QBC939 cell growth and the ability to metastasize could be suppressed by LPS. CXCR 4 gene could become a promising therapeutic device for cholangiocarcinoma.