Abstract: Objective: To explore the relationship of hTERT and CD 44v6 expression with the carcinogenesis, infiltra-tion, metastasis, and prognosis of colorectal carcinoma. Methods:Immunohistochemistry was used to detect the expres -sion of hTERT and CD44v6 in the tissue of colorectal carcinoma and adenoma. Results: The positive rates of expression of hTERT and CD 44v6 in colorectal carcinoma tissue were 70.73% and 10.53%, respectively; the positive rates of expression of hTERT and CD 44v6 were10.53% and 10.53% in colorectal adenoma tissue, respectively, with a significant difference be-tween the 2 groups ( P<0.01). The positive rates of expression of hTERT and CD 44v6 in colorectal carcinoma tissue from patients with lymph node metastasis were respectively 93.33% and 86.67%, distinctly higher than those from patients with-out lymph node metastasis ( 53.85% and 38.46%), with a significant difference (P<0.01). The positive rates of hTERT and CD44v6 expression in III and IV stage colorectal carcinoma tissue were 94.12% and 82.35%, higher than those in TNM I and II stage colorectal carcinoma tissue (54.17% and 33.33%), with a significant difference (P< 0.01). The positive rates of hTERT and CD44v6 expression in colorectal carcinoma patients with survival of less than 5 years were 90.91% and 77.27% , distinctly higher than those in patients with survival of5 or more years (P<0.01). Expression of hTERT and CD44v6 was not correlated with sex, age, tumor size, tumor location, or depth of infiltration (P>0.05). Conclusion:High ex-pressions of hTERT and CD 44v6 exist in colorectal carcinoma, especially in the tissue from patients with lymph node me-tastasis, TNM stages III and IV, and survival of fewer than 5 years. hTERT and CD 44v6 probably play an important role in the carcinogenesis, invasion, and metastasis of colorectal carcinoma. hTERT and CD 44v6 could be an index for the evalua-tion of infiltration, metastasis, and prognosis of colorectal carcinoma.