赵 琳, 史进方, 顾国浩, 蒋 敏, 陈永井, 张光波, 叶建新①, 陈卫昌①. 胃腺癌和相应癌旁组织中共信号分子ICOS/ICOSL mRNA表达的研究[J]. 中国肿瘤临床, 2010, 37(14): 792-795. DOI: 10.3969/j.issn.1000-8179.2010.14.004
引用本文: 赵 琳, 史进方, 顾国浩, 蒋 敏, 陈永井, 张光波, 叶建新①, 陈卫昌①. 胃腺癌和相应癌旁组织中共信号分子ICOS/ICOSL mRNA表达的研究[J]. 中国肿瘤临床, 2010, 37(14): 792-795. DOI: 10.3969/j.issn.1000-8179.2010.14.004
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ZHAO Lin1, SHI Jinfang1, GU Guohao, JIANG Min1, CHEN Yongjing1, ZHANG Guangbo1, YE Jianxin2, CHEN Weichang2. Study on Expression of Co-stimulatory Molecules ICOS/ICOSL mRNA in Gastric Adenocarcinoma and Homologous Paraneoplastic Tissue[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2010, 37(14): 792-795. DOI: 10.3969/j.issn.1000-8179.2010.14.004
Citation: ZHAO Lin1, SHI Jinfang1, GU Guohao, JIANG Min1, CHEN Yongjing1, ZHANG Guangbo1, YE Jianxin2, CHEN Weichang2. Study on Expression of Co-stimulatory Molecules ICOS/ICOSL mRNA in Gastric Adenocarcinoma and Homologous Paraneoplastic Tissue[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2010, 37(14): 792-795. DOI: 10.3969/j.issn.1000-8179.2010.14.004
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胃腺癌和相应癌旁组织中共信号分子ICOS/ICOSL mRNA表达的研究

Study on Expression of Co-stimulatory Molecules ICOS/ICOSL mRNA in Gastric Adenocarcinoma and Homologous Paraneoplastic Tissue

    摘要
  • 摘要: 目的：采用实时荧光定量PCR 技术的方法检测共信号分子ICOS及其配体ICOSL 在胃腺癌患者癌组织及相应癌旁组织中的基因表达量，以探讨共信号分子ICOS/ICOSL在胃腺癌发生、发展过程中所发挥的作用。方法：选择42例（年龄39～85岁）未接受放、化疗等抗癌治疗，并经病理切片确诊的胃腺癌初诊患者，收集患者手术切除的癌变组织及相应癌旁组织。提取每个组织标本中总RNA后，采用RT-PCR 法逆转录为cDNA ，再以cDNA 为模板，经Taqman探针PCR 方法扩增每个标本的ICOS/ICOSLcDNA 目的片段，得到组织标本中ICOS/ICOSL mRNA的绝对表达量（copies/mL）。 结果：胃腺癌及癌旁组织中ICOS及ICOSLmRNA 表达量在患者不同性别（2 组：男31例，女11例）、年龄（3 组：<60岁19例，60～70岁14例，>70岁9 例）、癌细胞分化程度（2组：高分化、中分化11例，低、中低分化31例）、肿瘤分期（4 组：Ⅰ期4 例，Ⅱ期5 例，Ⅲ期24例，Ⅳ期9 例）及淋巴结肿瘤转移（2 组：无转移7 例，有转移35例）等分组比较中均无统计学意义（P>0.05）；胃腺癌患者癌组织ICOSL mRNA 的表达量（4.39± 0.54Log copies/mL ）和癌旁组织ICOSL mRNA 的表达量（4.55± 0.47Log copies/mL ）相比无统计学意义（P>0.05），但胃腺癌癌组织中ICOS mRNA表达量（4.44± 0.62Log copies/mL ）和相应癌旁组织ICOS mRNA 表达量（4.76± 0.66Log copies/mL ）比较有显著统计学意义（P=0.005）。结论：胃腺癌组织中ICOS mRNA表达量明显低于相应癌旁组织，这可能与肿瘤的免疫逃逸有关。

     

    Abstract: Objective: To detect the gene expression of the co-stimulatory molecular ICOS/ICOSL mRNA in gastric adenocarcinomas and homologous paraneoplastic tissue using the technology of real-time fluorescent quantitative polymerase chain reaction (real-time fluorescence PCR), in order to explore the role of co-stimulatory molecules ICOS/ICOSL in the tumorigenesis and development of gastric adenocarcinoma. Methods:Forty-two gastric adenocarcinoma patients (age ranging 39-85 years), confirmed by pathologic section and without anticancer treatments such as radio-chemotherapy, etc., were selected as the research participants. The cDNA of tissue samples was synthesized by RT-PCR after extracting the total RNA, then the target sequences of ICOS/ICOSL cDNA were amplified by Taqman probe real-time fluorescence PCR using the template of tissue cDNA. The expression of ICOS/ICOSL mRNA of the gastric
    adenocarcinoma tissue and the adjacent tissue was quantitatively measured.Results: In the 42gastric carcinoma tissue samples, the relative mean expression of ICOS mRNA was higher than the adjacent tissue (P=0.005 ), but the difference of ICOSL mRNA between them had no statistical significance ( P>0.05).The expression of ICOSL mRNA of different sex, age, clinical staging and cellular differentiation of participants had no statistical significance ( P>0.05). Conclusion: The expression level of ICOS mRNA in gastric adenocarcinoma tissue was sharply reduced compared with their adjacent tissue and this may cause the immune system evasion of carcinomas.

     

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