Abstract:
Objective: To extract the crude polysaccharide from fresh apples to investigate its inhibitory effect on the proliferation of gastric adenocarcinoma SGC7901 cells in vitro. Methods:Crude polysaccharide was extracted from fresh apples using the alcohol precipitation method. Fresh apples were squeezed, the apple juice was dialyzed with pure water to exclude pigments, its protein was then eliminated using Sevage method. Alcohol was added into the juice to precipitate the crude polysaccharide, which was obtained after freeze-drying. The in vitro inhibitory effect on proliferation of gastric ade -nocarcinoma SGC7901cells of crude polysaccharide from apples was observed in different times and at different doses us -ing MTT method and the effect on colony formation of gastric adenocarcinoma SGC 7901 cells of crude polysaccharide from apples was assessed using colony-forming method. Results: The yield of crude polysaccharide was 0.1%. The crude polysaccharide (at0.5,0.1,0.02mg/mL) had different degrees of inhibitory effects on gastric adenocarcinoma SGC7901tu -mor cells in vitro in 48h (P<0.05) . The inhibitory rates of the crude polysaccharide at 0.1 mg/mL and0.5 mg/mL against SGC7901tumor cells were 10.8% and 18.9% ,respectively. Although, inhibitory effects were not significant when measured at 24h and 72h. This colony-forming experiment showed that with the increased administration of polysaccharide (at 0.02,0.1,0.5 mg/mL) the colony formation rate in turn showed a downward trend of 97.10± 2.33, 95.30± 3.62, and 93.6 ± 5.66. Conclusion: The crude polysaccharide of apples has a relatively strong inhibitory effect on gastric adenocarcinoma SGC7901tu -mor cells in vitro. A dose-effect relationship is evident.