Abstract:
Objective: To explore the association between HPV16E6 infection, the polymorphisms of HLA-DRB 1, DQB1 alleles and Kazakh esophageal squamous cell carcinomas in Xinjiang to further provide clues for Kazakh esopha-geal squamous cell carcinoma susceptibility genes. Methods:The HPV16E6 gene infection was detected by polymerase chain reaction (PCR) in 200 Kazakh patients with esophageal squamous cell carcinoma and150 healthy controls . The polymorphisms of HLA-DRB 1DRB1*1501 and HLA-DQB 1*0602 were typed by sequence specific primer by using poly-merase chain reaction (PCR-SSP)in200 Kazakh patients with esophageal squamous cell carcinoma and150 healthy con-trols. Results: Of the patients of Kazakhstan with esophageal squamous cell carcinoma, HPV 16E6 infection rate was 41%, significantly higher than that of ethnic Kazakh population whose infection rate was within the normal range of 14% (P< 0.001 , OR= 3.94); no correlation between the normal controls with Kazakhstan HPV 16E6 infection rate and HLA-DRB 1*1501, DQB 1*0602 was found (P>0.05). Allele frequencies of HLA-DRB 1*1501 and HLA-DQB1*0602 of the patients from Kazakhstan with esophageal squamous cell carcinoma was higher than those in healthy controls (0.455 vs. 0.232 , P<0.001 , OR= 2.78; 0.69vs. 0.554 , P=0.006 , OR= 1.80, respectively). HLA DQB1*0602gene-positive allele in poorly differenti -ated squamous cell carcinoma group ( 68.8 percent) was higher than that of well-differentiated squamous cell carcinoma group (31.2% ), the difference was statistically significant (P<0.05). Conclusion:HPV16E6 infection may be an important factor causing squamous cell carcinoma in the Xinjiang Kazakhstan population. Individuals carrying HLA-DRB 1*1501 and/or DQB1*0602may be more susceptible to esophageal squamous cell carcinoma. HLA-DQB1*0602is related to the differentiation of esophageal squamous cell carcinoma in the Kazakhstani participants.