刘宇飞, 张兆祥, 韩 林, 胡余昌①, 陈 路①, 罗代珍①, 王 飞①. 乳腺癌中DLC1 和DLC1-mRNA 的表达及其临床意义[J]. 中国肿瘤临床, 2010, 37(15): 866-869. DOI: 10.3969/j.issn.1000-8179.2010.15.007
引用本文: 刘宇飞, 张兆祥, 韩 林, 胡余昌①, 陈 路①, 罗代珍①, 王 飞①. 乳腺癌中DLC1 和DLC1-mRNA 的表达及其临床意义[J]. 中国肿瘤临床, 2010, 37(15): 866-869. DOI: 10.3969/j.issn.1000-8179.2010.15.007
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LIU Yufei1, ZHANG Zhaoxiang1, HAN Lin1, HU Yuchang2, CHEN Lu2, LUO Daizhen2, WANG Fei2. Expression of DLC1 Protein and DLC1-mRNA in Breast Carcinoma and Their Clinical Significance[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2010, 37(15): 866-869. DOI: 10.3969/j.issn.1000-8179.2010.15.007
Citation: LIU Yufei1, ZHANG Zhaoxiang1, HAN Lin1, HU Yuchang2, CHEN Lu2, LUO Daizhen2, WANG Fei2. Expression of DLC1 Protein and DLC1-mRNA in Breast Carcinoma and Their Clinical Significance[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2010, 37(15): 866-869. DOI: 10.3969/j.issn.1000-8179.2010.15.007
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乳腺癌中DLC1 和DLC1-mRNA 的表达及其临床意义

Expression of DLC1 Protein and DLC1-mRNA in Breast Carcinoma and Their Clinical Significance

    摘要
  • 摘要: 目的：探讨乳腺癌中肝癌缺失基因1（deleted in liver cancer-1，DLC 1）和DLC 1-mRNA的表达及其临床意义。方法：应用原位杂交技术和免疫组织化学EnVision 二步法，检测52例乳腺浸润性导管癌和42例非癌乳腺组织（包括癌旁乳腺组织20例和乳腺纤维腺瘤22例）中DLC 1 和DLC 1-mRNA的表达，分析乳腺癌中DLC 1 和DLC 1-mRNA的表达与患者年龄、肿瘤最大直径、组织学分级、腋窝淋巴结转移和TNM分期等临床病理参数的相关性。结果：DLC 1 和DLC 1-mRNA在乳腺癌和非癌乳腺组织中的阳性表达率分别为（57.7% ，50.0%）和（92.9% ，90.5%），DLC 1 和DLC 1-mRNA在乳腺癌中的表达率显著低于非癌乳腺组织（χ2=14.717，P=0.000；χ2=17.518，P=0.000），差异均有显著统计学意义（P<0.001）。 乳腺癌中DLC 1 和DLC 1-mRNA的表达与组织学分级（rs=-0.811，P=0.000；rs=-0.422，P<0.05）、腋淋巴结转移（rs=-0.410，P<0.01；rs=-0.445，P<0.01）和TNM分期（rs=-0.319，P<0.05；rs=-0.405，P<0.05）均呈负相关；DLC 1-mRNA的表达与肿瘤最大直径（rs=-0.347，P<0.05）亦呈负相关。DLC 1 与肿瘤最大直径（rs=0.073）和DLC 1、DLC 1-mRNA的表达与患者年龄（rs=0.077；rs=0.008）的相关性均无统计学意义（P>0.05）。 结论：DLC 1 蛋白和DLC 1-mRNA在乳腺癌中呈低表达或失表达，在乳腺癌的恶性演进中扮演着重要角色。检测乳腺癌中DLC 1 和DLC 1-mRNA的表达，结合相关临床病理参数，可作为判断乳腺癌生物学行为和预后的一个参考指标。

     

    Abstract: Objective:To investigate the expression of DLC 1 and DLC1-mRNA and the correlation between these and cancerous and non-cancerous tissue of the breast. Methods:EnVision immunohistochemical method and in situ hybridiza -tion were used to detect the expression of DLC 1 protein and DLCI mRNA in52invasive breast ductal carcinomas and 42 non-cancerous mammary tissue samples, including 20paracancerous mammary tissue samples and 22mammary fibroad-enomas. The correlations between the expression of DLC 1 and DLC 1-mRNA in the breast carcinomas and clinicopathologi -cal parameters including patient age, maximum tumor size, tumor histological grade, axillary lymph node metastasis and TNM stage were analyzed.Results: The positive expression rates of DLC 1-mRNA and DLC 1 protein in the breast carcino -mas and non-cancerous breast tissue were (57.7% ,50.0% ) and(92.9% ,90.5% ), respectively. The positive expression rates of DLC 1 and DLC 1-mRNA were significantly lower in breast carcinomas than in non-cancerous mammary tissue (χ2=14.717 , P=0.000 ; χ2=17.518 , P=0.000 ). All the differences between the 2 groups had statistical significance ( P<0.001 ). Cor -relation analysis showed that the expression of DLC 1 protein and DLC1-mRNA in invasive breast ductal carcinomas were negatively correlated to tumor histological grade (rs=- 0.811 , P=0.000 ; rs=- 0.422 , P<0.05), axillary lymph node metastasis (r s=- 0.410 , P<0.01; rs=- 0.445 , P<0.01) and TNM stage (rs=- 0.319 , P<0.05; rs=- 0.405 , P<0.05). The expression of DLC1-mRNA in invasive ductal breast carcinomas was negatively correlated with tumor size (rs=- 0.347 , P<0.05). There was no significant correlation between the positive expression rate of DLC1 and tumor size (r s=0.073 , P>0.05). There were no significant correlations between the positive expression rates of DLC 1and DLC1-mRNA and patient age (r s=0.077 ; rs=0.008 ;P>0.05). Conclusion:Lower or no expression of DLC 1 and DLC 1-mRNA may play an important role in malignant pro -gression in breast carcinoma. Combining related clinicopathological parameters with detection of the expression of DLC1 and DLC 1-mRNA may be useful parameters for evaluating the biological behavior and the prognosis of breast carcinoma.

     

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