桑建荣, 陈永昌, 金 雯, 贾俊海, 车力龙. 一氧化氮对胃癌细胞AGS 生长的抑制及其机制探讨*[J]. 中国肿瘤临床, 2010, 37(18): 1032-1035. DOI: 10.3969/j.issn.1000-8179.2010.18.004
引用本文: 桑建荣, 陈永昌, 金 雯, 贾俊海, 车力龙. 一氧化氮对胃癌细胞AGS 生长的抑制及其机制探讨*[J]. 中国肿瘤临床, 2010, 37(18): 1032-1035. DOI: 10.3969/j.issn.1000-8179.2010.18.004
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SANG Jianrong, CHEN Yongchang, JIN Wen, JIA Junhai, CHE Lilong. Inhibitory Effect of Exogenous Nitric Oxide on Cell Growth of Gastric Cancer Cell Line AGS and Its Mechanisms[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2010, 37(18): 1032-1035. DOI: 10.3969/j.issn.1000-8179.2010.18.004
Citation: SANG Jianrong, CHEN Yongchang, JIN Wen, JIA Junhai, CHE Lilong. Inhibitory Effect of Exogenous Nitric Oxide on Cell Growth of Gastric Cancer Cell Line AGS and Its Mechanisms[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2010, 37(18): 1032-1035. DOI: 10.3969/j.issn.1000-8179.2010.18.004
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一氧化氮对胃癌细胞AGS 生长的抑制及其机制探讨*

Inhibitory Effect of Exogenous Nitric Oxide on Cell Growth of Gastric Cancer Cell Line AGS and Its Mechanisms

    摘要
  • 摘要: 目的：探讨外源一氧化氮（nitric oxide，NO）对胃癌AGS 细胞生长及c-jun和c-fos表达的影响。方法：应用MTT 法评价NO供体硝普钠（sodium nitroprusside，SNP ），SNP 的同源类似物铁氰化钠Na3Fe（CN）6 和一氧化氮合酶（nitric oxide synthase，NOS ）抑制剂N-硝基-L- 精氨酸甲酯（N-nitro-L-arginine methylester ，L-NAME ）对胃癌细胞生长的抑制作用，RT-PCR 和免疫印迹检测c-jun和c-fos的基因和蛋白表达。结果：SNP 剂量依赖性的抑制AGS 细胞的生长，其IC 50值为2.13mmol/L 。1mmol/L SNP 处理12、24、48h 后，细胞生长抑制率分别为（16.12± 0.85）% 、（20.33± 1.31）% 、（22.06± 2.03）%（P<0.05），NOS 抑制剂L-NAME 预处理明显减弱了SNP 对癌细胞的生长抑制效应，Na3Fe（CN）6 对胃癌细胞生长无抑制。与对照组比较，SNP 使c-jun和c-fos的mRNA 和蛋白表达降低，NOS 抑制剂L-NAME 预处理逆转了SNP 的这种作用，Na3Fe（CN）6 对c-jun和c-fos的mRNA 和蛋白表达无影响。结论：NO可诱导胃癌细胞c-jun和c-fos表达降低，抑制癌细胞生长。

     

    Abstract: Objective: To investigate the effect of exogenous nitric oxide on the growth and the expressions of c-jun and c-fos in gastric cancer cell line AGS. Methods:The AGS cells were treated with SNP, Na 3Fe(CN)6 and L-NAME. The growth inhibition of cancer cells was determined by MTT assay. The changes of mRNA expressions of c-jun and c-fos were examined by RT-PCR. The protein expressions of c-jun and c-fos were analyzed by Western blot. Results: SNP inhibited AGS cell growth in a dose-dependent manner with a 24hour IC50value of 2.13mmol/L. When the AGS cells were treated with 1 mmol/L SNP for12, 24and 48hours, the growth inhibition rates were ( 16.12± 0.85)%, (20.33± 1.31)% and ( 22.06± 2.03)%, respectively ( P<0.05). L-NAME reversed the effect of SNP in inhibiting the growth of AGS cells. Na 3Fe(CN)6 had a very small effect on the growth inhibition of AGS cells. Compared with the control group, SNP reduced the gene and protein expressions of c-jun and c-fos in gastric cancer cell line AGS. Such decreases were reversed by pretreatment with L-NAME. Na 3Fe(CN)6 had little effect on the gene and protein expressions of c-fos and c-jun. Conclusion : Nitric oxide may work to inhibit gastric cancer cell line AGS growth by decreasing the expression of c-fos and c-jun.

     

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