Abstract: Objective: To study the molecular mechanisms of adverse psychological stress on ovarian cancer tumori -genesis. Methods:proteomics methodwas employed to locate and identify differentially expressed proteins in ovarian can-cer-bearing nude mice xenograft model of adverse psychological stress, to be verified using Western Blot. The expression of nm23protein and NDRG1 protein were detected by an immunohistochemical SP method in transplanted tumor tissue in-cluding 25cases in the stress group and 20cases in the control group. Results: At pI7.1, Mr = 21ku, and pI 5.5, Mr = 43 ku, there are two notable points of difference, the previous expression was increased in the stress group while another had decreased expression, they were identified as nm23protein and NDRG1 protein by mass spectrometry; the Western Blot-ting results were consistent with those of proteomics method; and the results of immunohistochemistry showed that the pos -itive rate of nm 23in the stress group and control group were 100 % and 70%, respectively, while rates of NDRG1 protein were56% and 85%, respectively, the differences were significant (P<0.05). Conclusion:Adverse psychological stress may influence the development of tumors by activation of oncogenes and inactivation of tumor suppressor genes, and nm 23 and NDRG 1 could be used as a genetic research and gene therapy target.