HA14-1 联合环磷酰胺调控肺癌小鼠Bcl-2、Bax蛋白的表达*

谭明旗; 孙惠梅; 边明艳

doi:10.3969/j.issn.1000-8179.2010.19.002

HA14-1 联合环磷酰胺调控肺癌小鼠Bcl-2、Bax蛋白的表达*

HA14-1 Combined with Cyclophosphamide Modulates the Expression of Bcl-2 and Bax in Lung Cancer in Mice

摘要

摘要: 目的：探讨HA14-1 联合环磷酰胺对肺癌小鼠Bcl- 2、Bax 蛋白的表达。方法：建立小鼠肿瘤模型，分为生理盐水组、HA14-1 组、环磷酰胺组和HA14-1 + 环磷酰胺组，应用Western blot和免疫组化方法检测Bcl- 2、Bax 的蛋白表达。结果：HA14-1组、环磷酰胺组、HA14-1 + 环磷酰胺组较生理盐水组Bcl- 2 表达减少、Bax 表达增加（P<0.05），HA14-1 + 环磷酰胺组较单一的HA14-1 组或环磷酰胺组Bcl- 2 表达明显减少、Bax 表达明显增加（P<0.05）。结论：HA14-1 可以通过抑制Bcl- 2 的表达，增加Bax的表达，从而促进肿瘤细胞的凋亡，同时增强环磷酰胺的抗肿瘤特性。

Abstract: Objective: To explore the effect of HA14-1 combined with cyclophosphamide (CTX) on the expression of Bcl- 2 and Bax proteins in lung cancer in mice. Methods: Mouse models of lung carcinoma were established and divided in-to 4 groups: the HA 14-1 group, the CTX group, the combination HA14-1 +CTX group and the normal saline group used as the control. Western blot and immunohistochemistry were applied to detect the expression of Bcl-2 and Bax.Results: The HA14-1 group, the CTX group and the HA14-1 +CTX group had lower Bcl-2 protein expression and higher Bax expression compared with the control group (P<0.05). The HA 14-1 +CTX group had the lowest Bcl-2 protein expression and the high-est Bax protein expression ( P<0.05). Conclusion:HA14-1 can promote tumor cell apoptosis and enhance the efficacy of CTX chemotherapy by downregulating Bcl-2 expression and upregulating Bax expression.

HTML全文

参考文献(0)

施引文献

资源附件(0)