Abstract: Objective: To detect the level of Twist, P53, and E-cadherin protein expression in human esophageal can-cer tissue, adjacent tissue and normal esophageal tissue, and to determine if there is a relevant relationship between Twist, P53, and E-cadherin protein expression and the clinicopathological parameters of esophageal cancer. Methods:Research-ers collected 30samples of esophageal cancer tissue and adjacent tissue and 10normal esophageal tissue samples from patients with benign esophageal disease. Immunohistochemistry and Western blot were used to detect the expression of Twist, P53and E-cadherin. The results were analyzed by SPSS 15.0. Results: The expression of Twist and P 53was higher in esophageal cancer tissue than in adjacent tissue and normal esophageal tissue. E-cadherin expression was lower in esophageal cancer tissue than in adjacent tissue and normal esophageal tissue. No significant difference was found in Twist, P53and E-cadherin expression between adjacent tissue and normal esophageal cancer tissue. Twist protein expres-sion was significantly correlated with depth of invasion, lymph node metastasis, TNM stage and tumor differentiation. Conclusion: Twist may inhibit cell apoptosis through regulation of the ARF/P 53pathway and can affect E-cadherin expression, promoting the epithelial-mesenchymal transition (EMT) and promoting the occurrence and metastasis of esophageal can-cer. Twist can be used as a specific index for the diagnosis and prognostic evaluation of esophageal cancer.