Abstract: Objective:To investigate the expression and significance of EZH 2 and PTEN in bladder transitional cell car -cinoma (BTCC) and ascertain whether such expression is related to disease-free survival in patients with BTCC. Methods: The expression of EZH2 and PTEN was detected in a tumor tissue microarray consisting of 80transitional cell carcinomas and 10normal bladder mucosal tissues using immunohistochemistry. The predictive value of this expression for prognosis was investigated by Kaplan-Meier curves and Cox proportional hazards analysis in a multivariate model.Results: Expres-sion of EZH2 and PTEN protein were detectable in 80.0% and 45.0% of bladder cancer specimens, respectively. The posi-tive expression rates of EZH 2 and PTEN showed a statistically significant difference in BTCC groups with different clinical stages and pathological grades (P<0.05). There was a negative correlation between EZH2 and PTEN expression ( P=0.033 ). The mean postoperative disease-free survival time in BTCC patients was 39.4 months. The 1-, 3- and 5-year dis -ease-free survival rates for the study population were70.0%,55.2% and 41.4%, respectively. Univariate analysis showed that pathological grade, tumor number and EZH 2 expression were all significant prognostic factors. Multivariate analysis al-so identified pathological grade, tumor number and EZH2 expression as independent risk factors for disease-free survival. Conclusion:This retrospective study found that abnormal expression of EZH 2 and PTEN may play an important role in the development and progression of BTCC. Additionally, expression of EZH2, as well as pathological grade and tumor number is a useful parameter for making a prognosis in patients with BTCC.