黄宇琨, 邱 氟①. 聚肌胞联合E7（44-62）对宫颈癌患者树突状细胞的影响*[J]. 中国肿瘤临床, 2010, 37(22): 1265-1267. DOI: 10.3969/j.issn.1000-8179.2010.22.002
引用本文: 黄宇琨, 邱 氟①. 聚肌胞联合E7（44-62）对宫颈癌患者树突状细胞的影响*[J]. 中国肿瘤临床, 2010, 37(22): 1265-1267. DOI: 10.3969/j.issn.1000-8179.2010.22.002
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HUANG Yukun1, QIU Fu2. The Effect of Poly (I:C) Stimulation in Conjunction with HPV E7 (44-62) Treatment on Dendritic Cells in Cervical Cancer Patients[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2010, 37(22): 1265-1267. DOI: 10.3969/j.issn.1000-8179.2010.22.002
Citation: HUANG Yukun1, QIU Fu2. The Effect of Poly (I:C) Stimulation in Conjunction with HPV E7 (44-62) Treatment on Dendritic Cells in Cervical Cancer Patients[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2010, 37(22): 1265-1267. DOI: 10.3969/j.issn.1000-8179.2010.22.002
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聚肌胞联合E7（44-62）对宫颈癌患者树突状细胞的影响*

The Effect of Poly (I:C) Stimulation in Conjunction with HPV E7 (44-62) Treatment on Dendritic Cells in Cervical Cancer Patients

    摘要
  • 摘要: 目的：探讨聚肌胞联合E7（44-62）刺激对宫颈癌患者外周血树突状细胞的影响。方法：分离和培养正常对照组（C组）、宫颈癌组和癌前病变组（宫颈上皮内瘤变Ⅱ/Ⅲ期，CIN 组）外周血的树突状细胞，分别给予聚肌胞或聚肌胞联合E7（44-62）刺激；检测刺激前后的树突状细胞的增殖反应以及CD11c+/CD 86+的表达水平。结果：1）宫颈癌组外周血的树突状细胞占外周血单核细胞的比例（0.12+ 0.007）% 显著低于C 组（1.13+ 0.056）% 和CIN 组（0.25+ 0.012）% ，P 均<0.01；CIN 组树突状细胞的比例显著高于宫颈癌组（P<0.05）。 2）与聚肌胞比较，聚肌胞联合E7（44-62）刺激能够显著提高C 组、CIN 组和宫颈癌患者外周血的树突状细胞的增殖反应（P 均<0.01）。 C 组树突状细胞对聚肌胞联合E7（44-62）刺激的增殖反应显著高于宫颈癌组和CIN 组（P 均<0.01）。 3）宫颈癌组树突状细胞的CD11c+/CD 86+的表达水平显著低于C 组和CIN 组（P<0.01，<0.05），C 组CD11c+/CD 86+的表达水平显著高于CIN 组（P<0.05）。 4）与聚肌胞比较，聚肌胞联合E7（44-62）刺激能够显著提高C 组、CIN 组和宫颈癌患者外周血的树突状细胞的CD11c+/CD 86+的表达水平，P 值分别<0.01，<0.05，<0.05。结论：聚肌胞联合E7（44-62）通过提高宫颈癌患者外周血树突状细胞的增殖功能，同时促进其活化反应，从而增强免疫功能。

     

    Abstract: Objective:To investigate the effect of poly (I:C) stimulation in conjunction with HPV E7 (44-62) on dendritic cells in cervical cancer patients. Methods:Human dendritic cells were taken from patients with cervical cancer, normal pa-tients, and patients with precancerous lesions (cervical intraepithelial neoplasia stage Ⅱ/Ⅲ). The proliferation of dendritic cells and the expression of CD 11c +/CD 86+were measured after the cells were cultured in medium containing poly (I:C) or poly (I:C) and E7 (44-62). Results: (1) Samples from the patients with cervical cancer had significantly lower percentages of dendritic cells ( 0.12% +0.007 % ) in peripheral blood mononuclear cells than samples from the normal controls (1.13% + 0.056 %) and the samples from patients with precancerous lesions (0.25%+0.012 %) (P<0.01). The percentage of dendritic cells in peripheral blood mononuclear cells from the CIN group was significantly higher than that in the cervical cancer group (P<0.05). (2) Compared with poly (I:C) alone, poly (I:C) and E7 (44-62) stimulation significantly enhanced the prolifer -ation of dendritic cells in the normal group, the CIN group and the cervical cancer group ( P<0.01). The proliferative re -sponse of dendritic cells to poly (I:C) and E 7 (44-62) in the normal group was significantly higher than that in the CIN and cervical cancer groups ( P<0.01). (3) The expression of CD 11c +/ CD86+ in dendritic cells from the cervical cancer group was significantly lower than that in the normal (P<0.01) and CIN groups ( P<0.05). The expression of CD 11c+/ CD86+ in dendritic cells in the control group was significantly higher than that in the CIN group (P<0.05). (4) Compared with poly(I:C) alone, poly (I:C) and E7 (44-62) together significantly increased CD11c+/CD 86+ expression in dendritic cells in the normal group (P<0.01), the CIN group ( P<0.05) and the cervical cancer group ( P<0.05). Conclusion:Poly(I:C) and HPV E7 (44 62) together can enhance the immune function of dendritic cells in cervical cancer patients by increasing the proliferation of dendritic cells and promoting their activation.

     

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