Abstract:
Objective: To investigate the protection of joint application of gonadotropin-releasing hormone antagonist (GnRH Antagonist) and estrogen in preventing cisplatin -induced ovarian failure in mice model. Methods:Forty eugamic fe-male BALB/c mice with an age range from 5 to 6 weeks and bodyweight of (18± 3) g were randomly divided into four groups: the blank control, cisplatin(DDP), GnRHant(cetrorelix) + estrogen(progynova), and cetrorelix + progynova + DDP group, with10in each group. The general conditions, such as bodyweight change, ovary and uterus wet weight in the groups, were observed and compared after administration, and the numbers of the follicle and histomorphological features of ovary were compared. Radioimmunoassay was used to analyze the serum level of follicle-stimulating hormone (FSH) and estradiol (E2) among the four groups. Results: The concentrations of serum FSH and E2 were ( 1.78± 0.23) IU/L and (37.65± 1.77) pg/mL in the DDP group, and were (1.22± 0.13) IU/L and (50.43± 2.70) pg/ml in cetrorelix + progynova+DDP group, respectively. There were significant differences between the two groups (P<0.05). The average weights of ovarian and uterus were ( 8.87± 0.69) mg and ( 53.80± 13.65) mg in DDP group respectively, which were significantly lower compared to ( 17.19± 0.61) mg and ( 66.97± 8.30) mg in cetrorelix + progynova+DDP group ( P<0.05). The total number of follicle was 471 ± 29.71in DDP group, which was significantly lower compared to 815 ± 25.95in cetrorelix + progynova+DDP group ( P< 0.05). There was statistical significance in the differences between the two groups. Conclusion:GnRHant in combination with estrogen could reduce the chemotherapy-induced ovarian damage in mice, and protect ovarian reserve of mice during the treatment.