李书军, 和宇峥, 闫红江, 沈玉光①, 张 逊①. siRNA 沉默artemin 蛋白对食管癌TE11细胞侵袭能力的影响[J]. 中国肿瘤临床, 2010, 37(24): 1432-1436. DOI: 10.3969/j.issn.1000-8179.2010.24.015
引用本文: 李书军, 和宇峥, 闫红江, 沈玉光①, 张 逊①. siRNA 沉默artemin 蛋白对食管癌TE11细胞侵袭能力的影响[J]. 中国肿瘤临床, 2010, 37(24): 1432-1436. DOI: 10.3969/j.issn.1000-8179.2010.24.015
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LI Shujun1, HE Yuzheng1, YAN Hongjiang1, SHEN Yuguang2, ZHANG Xun2. The Effect of siRNA Targeting the Artemin Gene on the Invasiveness of Esophageal Carcinoma[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2010, 37(24): 1432-1436. DOI: 10.3969/j.issn.1000-8179.2010.24.015
Citation: LI Shujun1, HE Yuzheng1, YAN Hongjiang1, SHEN Yuguang2, ZHANG Xun2. The Effect of siRNA Targeting the Artemin Gene on the Invasiveness of Esophageal Carcinoma[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2010, 37(24): 1432-1436. DOI: 10.3969/j.issn.1000-8179.2010.24.015
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siRNA 沉默artemin 蛋白对食管癌TE11细胞侵袭能力的影响

The Effect of siRNA Targeting the Artemin Gene on the Invasiveness of Esophageal Carcinoma

    摘要
  • 摘要: 目的：通过siRNA 干扰技术沉默ARTN 基因在人食管癌细胞TE11中的表达，研究其对人食管癌细胞增殖、迁移和侵袭能力的影响。方法：设计并合成3 条特异性针对ARTN 基因的siRNA ，构建了真核表达载体pSilencer 2.1-ARTN-siRNA，转染到TE11细胞中，经Real-time PCR和Western blot检测ARTN 在mRNA 和蛋白水平的表达；通过MTT 比色法、细胞划痕实验和Boyden小室侵袭实验观察转染pSilencer 2.1-ARTN-siRNA 后细胞增殖、迁移和侵袭能力的改变。结果：转染重组质粒pSilencer 2.1-ARTN-siRNA后人食管癌细胞TE11中ARTN 的mRNA 表达受到抑制，蛋白表达降低；通过体外实验表明，细胞的增殖能力、迁移能力和侵袭能力均减弱。结论：siRNA 沉默ARTN 基因的表达可降低人食管癌细胞TE11的增殖、迁移和侵袭能力，ARTN 可能是食管癌的一个生物治疗靶点，有望成为治疗食管癌的新途径。

     

    Abstract: Objective: To investigate whether ARTN protein can affect proliferation, migration and invasion of esopha -geal carcinoma cell line TE11using small interfering RNA. Methods:Three pairs of small interfering RNA (siRNA) specific to ARTN mRNA were designed and cloned into plasmid pSilencer 2.1-U6-neo. The recombined eukaryotic expression vec -tor pSilencer 2.1-ARTN-siRNA was then transfected into TE 11 cells. The levels of ARTN mRNA and protein expression were detected using real-time PCR and Western blotting. The proliferation, migration and invasion of TE 11-pSilencer 2.1 cells and TE 11-pSilencer 2.1-ARTN-siRNA cells were assessed by MTT, wound healing and Boyden chamber. Results: The recombined eukaryotic expression vector pSilencer2.1-ARTN-siRNA was transfected successfully. After transfection, the levels of expression of ARTN mRNA and protein were significantly reduced in TE 11- pSilencer 2.1-siRNA-ARTN cells. The proliferation, migration and invasion of TE 11-pSilencer 2.1-siRNA-ARTN cells were also significantly decreased. Conclusion: Silencing the ARTN gene using siRNA can effectively suppress proliferation, migration and invasion of TE11esophageal carcinoma cells. ARTN may be a potential therapeutic target, and silencing the ARTN gene using siRNA may provide a nov-el therapeutic approach for esophageal cancer.

     

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