Abstract: investigate the expression and clinical significance of the pituitary tumor transformed gene ( PTTG ), the basic fibroblast growth factor ( bFGF ), and the vascular endothelial growth factor-C ( VEGF-C ) in the occurrence and progression of human colorectal carcinoma ( CC ). The relationship between these indices and clinicopathologic factors was also examined. Methods: Reverse transcriptase-polymerase chain reaction ( RT-PCR ) was used to determine the mRNA expression of PTTG. Streptavidin peroxidase ( SP ) immunohistochemical staining was performed to assay the expression of the three proteins in 20 normal mucosa ( NM ) and 80 CC tissues. Results: The PTTG, bFGF, and VEGF-C expression levels in CC were significantly higher than those in NM ( P < 0.01, P < 0.01, and P < 0.01, respectively ), and were positively correlated with the depth of tumor invasion, lymph node metastasis, and Duke's staging. Significantly positive correlations were observed among PTTG, b-FGF, and VEGF-C in CC tissues. Conclusion: The high PTTG, bFGF, and VEGF-C expression may be significantly involved in the onset and progression of CC, and can be used as a predictive and prognostic factor. Interactions between PTTG, bFGF, and VEGF-C may promote CC progression.