Abstract:
To investigate the promoter methylation status of SFRP1, SFRP2, SFRP4, and SFRP5 genes in renal clear cell carcinoma ( CCRCC ) as well as to discuss the relationship between the methylation of genes and development of CCRCC. Methods: Methylation-specific polymerase chain reaction method was used to examine the methylation status of the 5' CpG island of SFRP1, SFRP2, SFRP4, and SFRP5 genes in 66 cases with CCRCC and 30 with paraneoplastic tissues. Related clinical data were analyzed. Results: Methylation frequency rates of SFRP1, SFRP2, SFRP4, and SFRP5 in CCRCC were 77.3% ( 51/66 ), 72.7% ( 48/66 ), 59.1% ( 39/66 ), and 69.7% ( 46/66 ), respectively, significantly higher than those in the paraneoplastic tissues ( P < 0.05 ). Methylation status of SFRP1 and SFRP5 genes in CCRCC was related to TNM staging, whereas SFRP4 was correlated with tumor grading ( P < 0.05 ). Conclusion: Methylation of SFRP1, SFRP2, SFRP4, and SFRP5 genes may be involved in the morbidity of patients with CCRCC. SFRP1 and SFRP5 genes may be involved in the development, infiltration, and transfer of CCRCC. SFRP4 gene may be correlated with the malignant behaviors of CCRCC.