Abstract: To investigate the relationship between estrogen and the development of endometrial cancer. Methods: Real-time polymerase chain reaction ( PCR ) was used to detect VEGF, bFGF, and IL-8 mRNA expression in HEC-1A cells and an enzyme-linked immunosorbent assay ( ELISA ) was used to detect the VEGF, bFGF, and IL-8 proteins in the cell culture fluid after stimulation with 1 × 10-6 mol/L estradiol ( E2 group ) for 8 h or 12 h, and with 60 min pretreatment with 1 × 10-6 mol/L ER inhibitor ( ER group ) or 25 × 10-6 mol/L AKT inhibitor ( AKT group ) following stimulation with 1 × 10-6 mol/L estradiol for 8 h or 12 h. Western blot was used to detect AKT protein expression in the HEC-1A cells after stimulation with 1 × 10-6 mol/L estradiol for 15 min. Results: Real-time PCR and ELISA showed the mRNA and protein expression of VEGF, bFGF, and IL-8 in the E2 group was significantly higher than that in the control group ( P < 0.05 ). The mRNA and protein expression of VEGF, bFGF, and IL-8 in the ER group was significantly lower than that in the E2 group ( P < 0.05 ) except for the VEGF protein expression at 8 h and the IL-8 mRNA expression at 12 h. VEGF mRNA expression was slightly high in the E2 group at 12 h. The mRNA and protein expression of VEGF, bFGF, and IL-8 in the AKT group was significantly lower than that in the E2 group ( P < 0.05 ), except for IL-8 mRNA expression at 12 h. Western blot analysis showed that p-AKT protein expression in the HEC-1A cells after 15 min stimulation with 1 × 10-6 mol/L estradiol was markedly higher than that in the control group ( P < 0.05 ). Conclusion: Estrogen induced the production of VEGF, bFGF, and IL-8 through activating the AKT signal transmission pathway.