赵延大, 李菲菲, 任万华, 秦成勇. GRIM-19在肝细胞癌中的表达及与p-STAT3相关性的研究[J]. 中国肿瘤临床, 2011, 38(22): 1359-1362. DOI: 10.3969/j.issn.1000-8179.2011.22.001
引用本文: 赵延大, 李菲菲, 任万华, 秦成勇. GRIM-19在肝细胞癌中的表达及与p-STAT3相关性的研究[J]. 中国肿瘤临床, 2011, 38(22): 1359-1362. DOI: 10.3969/j.issn.1000-8179.2011.22.001
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Yanda ZHAO, Feifei LI, Wanhua REN, Chengyong QIN. Expression of GRIM-19 in Hepatocellular Carcinoma and Its Correlation with p-STAT3[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2011, 38(22): 1359-1362. DOI: 10.3969/j.issn.1000-8179.2011.22.001
Citation: Yanda ZHAO, Feifei LI, Wanhua REN, Chengyong QIN. Expression of GRIM-19 in Hepatocellular Carcinoma and Its Correlation with p-STAT3[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2011, 38(22): 1359-1362. DOI: 10.3969/j.issn.1000-8179.2011.22.001
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GRIM-19在肝细胞癌中的表达及与p-STAT3相关性的研究

Expression of GRIM-19 in Hepatocellular Carcinoma and Its Correlation with p-STAT3

    摘要
  • 摘要: 检测GRIM-19 mRNA、蛋白在肝细胞癌及非癌组织中的表达，探讨与p-STAT3的关系，及二者在肝细胞癌发生、发展中的作用。方法：免疫组化检测10例肝细胞癌及非癌组织中GRIM-19蛋白、p-STAT3蛋白的定位，RT-PCR和Westernblot检测40例肝细胞癌及非癌组织中GRIM-19 mRNA和GRIM-19蛋白、p-STAT3蛋白表达。分析GRIM-19与临床病理特征的关系，及与p-STAT3的相关性。结果：在肝细胞癌及非癌组织中，GRIM-19、p-STAT3分别定位于胞浆、胞核。GRIM-19 mRNA在肝细胞癌的表达（0.40±0.31）低于非癌组织（0.56±0.67）（P<0.05），GRIM-19蛋白与mRNA表达一致。p-STAT3蛋白在肝细胞癌的表达（0.92±1.40）高于非癌组织（0.24±0.22）（P<0.05）。GRIM-19 mRNA表达与临床分期、门脉癌栓有关，与性别、年龄、AFP、肿瘤大小、肝硬化、转移、组织分级无关，GRIM-19蛋白有同样的趋势。GRIM-19与p-STAT3负相关（r=-0.55，P<0.05）。结论：GRIM-19低表达可能与肝细胞癌发生、发展、侵袭等有关，并与p-STAT3共同促进肝细胞癌发生、发展。

     

    Abstract: ogy, Provincial Hospital Affiliated to Shandong University, Ji'nan 250021, China
     Abstract　Objective: To examine the expression of GRIM-19 mRNA and protein in hepatocellular carcinoma ( HCC ) tissue and homologous noncancerous liver tissue, to explore the correlation between GRIM-19 and p-STAT3 expression, and to analyze the functions of these proteins in the oncogenesis and progression of HCC. Methods: Immunohistochemistry was used to detect the cellular distribution of GRIM-19 and p-STAT3 proteins in 10 HCC and 10 noncancerous liver tissue samples. Reverse transcription-polymerase chain reaction ( RT-PCR ) and Western blot were used to determine the expression of GRIM-19 mRNA, GRIM-19 protein, and p-STAT3 protein in 40 specimens with HCC and corresponding noncancerous tissue of the liver. The relationship between GRIM-19 and various clinicopathologic features and the correlation between GRIM-19 and p-STAT3 were statistically analyzed. Results: In the HCC and noncancerous liver tissue, GRIM-19 protein was located in the cytoplasm, and P-STAT3 protein was located in the nuclei. The expression of GRIM-19 mRNA was significantly lower in HCC ( 0.40 ± 0.31 ) than in the corresponding noncancerous liver tissue ( 0.56 ± 0.67, P < 0.05 ), while the expression of GRIM-19 protein was consistent with the expression of GRIM-19 mRNA. By contrast, the expression of p-STAT3 protein was markedly higher in HCC ( 0.92 ± 1.40 ) than in the noncancerous liver tissue ( 0.24 ± 0.22, P < 0.05 ). The expression of GRIM-19 mRNA was correlated with the clinical stage and pylic tumor embolus, but not with gender, age, AFP, tumor size, liver cirrhosis, metastasis, or histologic grade. A similar trend was observed between these parameters and GRIM-19 protein expression. There was a negative correlation between the expression levels of GRIM-19 and p-STAT3 in HCC ( r = -0.55, P < 0.05 ). Conclusion: Low erexpression of GRIM-19 in HCC may be associated with oncogenesis, progression, and invasion. Both GRIM-19 and p-STAT3 may promote the occurrence and development of liver tumors.

     

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