王雷, 单保恩, 刘亮, 孟宪利, 王士杰. IL-27基因修饰树突状细胞活化的特异性CTL对人食管癌裸鼠移植瘤生长增殖的影响[J]. 中国肿瘤临床, 2011, 38(23): 1419-1423. DOI: 10.3969/j.issn.1000-8179.2011.23.001
引用本文: 王雷, 单保恩, 刘亮, 孟宪利, 王士杰. IL-27基因修饰树突状细胞活化的特异性CTL对人食管癌裸鼠移植瘤生长增殖的影响[J]. 中国肿瘤临床, 2011, 38(23): 1419-1423. DOI: 10.3969/j.issn.1000-8179.2011.23.001
shu
Lei WANG, Baoen SHAN, Liang LIU, Xianli MENG, Shijie WANG. Effects of Specific CTL Activated by Interleukin-27 Gene-modified DC on Growth of Transplanted Tumor of Human Esophageal Carcinoma in Nude Mice[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2011, 38(23): 1419-1423. DOI: 10.3969/j.issn.1000-8179.2011.23.001
Citation: Lei WANG, Baoen SHAN, Liang LIU, Xianli MENG, Shijie WANG. Effects of Specific CTL Activated by Interleukin-27 Gene-modified DC on Growth of Transplanted Tumor of Human Esophageal Carcinoma in Nude Mice[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2011, 38(23): 1419-1423. DOI: 10.3969/j.issn.1000-8179.2011.23.001
shu

IL-27基因修饰树突状细胞活化的特异性CTL对人食管癌裸鼠移植瘤生长增殖的影响

Effects of Specific CTL Activated by Interleukin-27 Gene-modified DC on Growth of Transplanted Tumor of Human Esophageal Carcinoma in Nude Mice

    摘要
  • 摘要: 研究IL-27基因转染树突状细胞（DC）体内诱导免疫杀伤食管癌细胞的效能及其机制。方法：基因转染的方法建立表达IL-27基因的树突状细胞（DC/IL-27）；构建人食管癌细胞裸鼠移植瘤模型，皮下注射食管癌细胞抗原致敏、IL-27基因修饰DC（DC/IL-27-Ag）活化的特异性CTL后观察荷瘤裸鼠抑瘤率；TUNEL法检测荷瘤裸鼠肿瘤细胞的原位凋亡；流式细胞术检测移植瘤细胞的细胞周期、凋亡率。结果：RT-PCR显示DC/IL-27细胞中有IL-27 p28和EBI3亚基基因表达提示转染成功；免疫接种DC/IL-27-Ag活化的CTL组的抑瘤率为58.28%，明显高于其他组，差异有统计学意义（P<0.01）。TUNEL法检测显示，DC/IL-27-Ag活化的CTL组凋亡率明显高于其他组（P<0.01），流式细胞术（FCM）显示肿瘤组织内细胞增殖指数为（23.92±1.60）%，显著低于其他组，差异有统计学意义（P<0.01）；细胞凋亡率为（32.78±0.83）%，显著高于其他组，差异有统计学意义（P<0.01）。结论：DC/IL-27-Ag可活化特异性CTL，在裸鼠体内产生了抑制肿瘤生长的作用，显著抑制食管癌细胞增殖、促进其凋亡，为DC应用于食管癌的免疫治疗提供了理论和动物实验依据。

     

    Abstract: To investigate the effects of the specific CTL activated by IL-27 gene-modified DC on the growth of transplantated tumor of human esophageal carcinoma and to study the effects of IL-27 on cell apoptosis and its mechanisms. Methods: The human DC/IL-27 cells secreting IL-27 were successfully obtained using gene transfection. After the nude mice models of transplantated tumor of human esophageal carcinoma were established, the mice were immunized with specific CTL activated by IL-27 gene-modified DC loaded with esophageal tumor lysate ( DC/IL-27-Ag ). The volume and mass of the transplantated tumors were measured. The cell apoptosis in the tumor was determined by TUNEL. The cell cycle and apoptotic rate of transplantated tumors at G0/G1, S and G2/M stages were assayed by flow cytometry. Results: RT-PCR showed the gene expression of IL-27 p28 and EBI3 subgenes in DC/IL-27 cells, and it proved the success of the gene transfection. The inhibitory rate of specific CTL activated by DC/IL-27-Ag was 58.28%, which was higher than that in other group ( P < 0.01 ). TUNEL also showed the morphology of cell apoptosis with specific CTL activated by DC/IL-27-Ag had typical changes in apoptosis than that in other group, the difference was statistically significant ( P < 0.01 ). FCM showed the proliferation index of transplanted tumor tissue with specific CTL activated by DC/IL-27-Ag was ( 23.92 ± 1.60 ) % , which was lower than that in other group, the difference was statistically significant ( P < 0.01 ). The ratio of apoptotic cells of transplanted tumors with specific CTL activated by DC/IL-27-Ag was ( 32.78 ± 0.83 ) %, and the value was higher than that in other group, the difference was statistically significant ( P < 0.01 ). Conclusion: Specific CTL activated by DC/IL-27-Ag may inhibit the tumor formation in nude mice, obviously inhibit the cell proliferation and enhance the apoptosis of esophageal carcinoma cells in vivo, which provides theoretical and experimental foundation for the application of DC in immunotherapy of esophageal carcinoma.

     

    • HTML全文
    • 参考文献(0)
    • 相关文章
    • 施引文献
  • 资源附件(0)

/

返回文章
返回