Abstract: To evaluate the association between the polymorphism in the DNA promoter region and the risk of lung cancer in the Chinese population. Methods: The promoter region of the PIM1 gene was amplified through polymerase chain reaction ( PCR ) and sequenced in 25 randomly selected control subjects and 25 lung cancer patients. The PIM1 -1882A > T polymorphism was done via PCR and sequenced in 206 lung cancer patients and 189 control subjects. A case-control study was performed to evaluate the association between polymorphism and lung cancer risk. Results: The allele frequency of -1882 A > T of patients and controls was 6.8% versus 11%, respectively, and a significant difference between allele frequencies in lung cancer patients and healthy control patients was observed ( P = 0.025 ). Relative risk analysis showed that the genotype of AT+ TT vs. AA decreased the risk of developing non-small-cell lung cancer ( NSCLC ) ( OR = 0.568, 95%CI = 0.335-0.962, P = 0.034 ). Conclusion: The PIM1 SNP ( -1882 A > T ) was associated with the NSCLC risk in the Chinese population, and the PIM1 -1882 A > T polymorphism may help in reducing the risk of lung cancer.