Abstract: To analyze the distribution of Epstien-Barr virus ( EBV ) antibodies, EBNA1/IgA and VCA/IgA, in people of different ages or genders, and to understand the probability of suffering from nasopharyngeal carcinoma ( NPC ) in people aged from 30 years to 59 years, through a survey of 16 to 355 persons in high-incidence areas of NPC. Methods: ELISA was used to detect the serum levels of EBNA1/IgA and VCA/IgA. The positive rates of antibodies and the probability of suffering from NPC in different population were calculated, and the characteristics of EBV antibodies from different aspects were analyzed. Results: The positive rates of EBNA1/IgA in the total population ( in males and in females ) were 4.04%, 5.11%, and 3.23%,  respectively.  The positive rate of EBNA1/IgA in males tended to increase ( P < 0.05 ), except for the 30 to 39 age-brackets compared with the females. The positive rates of VCA/IgA in total population ( in males and in females ) were 5.84%, 5.97%, and 5.74%, respectively. The positive rate of VCA/IgA was unrelated to the gender ( P > 0.05 ) in 35 to 59 age-brackets. The positive rates of EBNA1/IgA and VCA/IgA in males were positively associated with the age of the respondents ( P < 0.05 ). The distributions of the two positive rates of antibodies, especially EBNA1/IgA, as well as the incidence of NPC in different age-brackets had good consistency. No significant association between the above indices and the ages in females were found. Conclusion: (1) EBNA1/IgA is related to gender for NPC screening in high-incidence areas. (2) The positive rates of EBNA1/IgA and VCA/IgA in males are positively associated with ages, whereas no significant association was observed between the above indices and the ages in females. (3) Good consistency was observed between the distribution of EBNA1/IgA positive rates and the NPC incidence in age-brackets. Finally, EBNA1/IgA may be ideal index for NPC screening.