Abstract: To study the parameters of 64-slice spiral computed tomography (CT) triple-phase enhanced scanning in evaluating lymphatic metastasis and its clinical application value. Methods: Patients diagnosed with gastric cancer were examined by 64-slice spiral CT before operation to measure gastric regional nodal involvement, and the results were compared with postoperative pathological findings. Valuable indicators on preoperative diagnosis of regional nodal involvement can be acquired by analyzing the parameters of lymphatic metastasis from the lymph node short diameter, lymph node CT value, lymph node short-to-long diameter ratio value, portal venous period, and flat sweep period of the differences of the CT value. Results: The lymph nodes portal venous period CT value ≥65 Hu, lymph node short diameter ≥6 mm, lymph node short aspect ratio ≥ 0.6, portal venous period, and flat sweep period of the differences of the CT value ≥35Hu were valuable parameters. A diagnosis sensitivity of 93.1% and specificity of 50% determined lymphatic involvement if two indices meet the above requirements. Moreover, if three parameters meet the requirements to evaluate lymphatic metastasis, the sensitivity was 73.5% and specificity of 75%. Conclusion: The use of 64-slice spiral CT triple-phase enhanced scanning and synthesis of various parameters of lymph nodes could lead to better judgment of perigastric lymph node metastasis, which can provide reference for preoperative, reasonable, and personalized treatment strategies for gastric cancer.