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摘要:
目的 分析Nanog在非小细胞肺癌（NSCLC）、胃腺癌、食管鳞状细胞癌中的表达特点及规律，探讨Nanog的表达在肿瘤发生、发展中的意义及其与肿瘤干细胞的关系。 方法 采用实时荧光定量PCR法分别检测78例NSCLC及其中10例肺癌患者的癌旁组织、39例胃腺癌及其中10例胃腺癌患者的癌旁组织和35例食管鳞癌及其中10例食管鳞癌患者的癌旁组织中Nanog的相对表达情况。 结果 NSCLC、胃腺癌和食管鳞癌组织中Nanog相对表达量明显高于对应的癌旁组织，且肺癌和胃腺癌与其对应的癌旁组织之间差异具有统计学意义（P < 0.05）。肺癌中Nanog的相对表达量与患者的临床分期和肿瘤组织学类型有关（P < 0.05）；胃腺癌组织中，Nanog的相对表达量与胃腺癌的临床分期、性别有关（P < 0.05）；食管鳞癌组织中，Nanog的相对表达量与淋巴结转移、临床分期有关（P < 0.05）。 结论 在肿瘤进展过程中，Nanog的表达与肿瘤的临床分期和淋巴结转移有关，提示Nanog的表达在腺癌的发生、发展中可能具有一定意义。 Abstract:Objective The current work aims to analyze the Nanog expression in tissue of several cancer types, explore its significance in the formation and development of tumor, and demonstrate its relationship with cancer stem cells. Methods Nanog was detected via real-time PCR in 78 non-small-cell lung cancer (NSCLC) tissue samples and 10 tissue samples adjacent to lung cance, 39 gastric adenocarcinoma tissue samples and 10 tissue samples adjacent to gastric adenocarcinoma, and 35 of esophageal squamous cell carcinoma tissue samples and 10 tissue samples adjacent to esophageal carcinoma. Results The Nanog expression was significantly higher in NSCLC, gastric adenocarcinoma, and esophageal squamous cell carcinoma tissue, with significant difference in NSCLC and gastric adenocarcinoma (P < 0.05). The Nanog expression was related with the clinical stage and tumor histological classification in NSCLC (P < 0.05), with the clinical stage and age in gastric adenocarcinoma (P < 0.05), and with the clinical stage and lymph-node metastasis in esophageal squamous cell carcinoma (P < 0.05). Conclusion The Nanog expression was related with clinical stage, lymph-node metastasis, and tumor histological classification, suggesting that it might be associated with the formation and development of adenocarcinoma. -
表 1 各种癌组织及其癌旁组织中Nanog相对表达量
Table 1. Relative expression of Nanog in tissue of several cancer types and corresponding adjacent tissue
表 2 肺癌组织中Nanog相对表达量与临床病理特征之间的关系
Table 2. Relationship between Nanog expression and clinicopathologic features of NSCLC
表 3 肺癌不同临床分期中Nanog相对表达量与癌旁组织之间的差异
Table 3. Relative expression of Nanog among NSCLC and adjacent tissue of different clinical stages
表 4 胃腺癌组织中Nanog相对表达量与临床病理特征之间的关系
Table 4. Relationship between Nanog expression and clinicogathologic features of gastric adenocarcinoma
表 5 食管鳞癌组织中Nanog相对表达量与临床病理特征之间的关系
Table 5. Relationship between Nanog expression and clinicopathologic features of esophageal squamous cell carcinoma
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