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摘要:
目的 探讨MUC2和MMP-9在乳腺癌中的表达及其与临床病理特征之间的关系。 方法 应用逆转录-聚合酶链反应（RT-PCR）方法检测68例乳腺良性疾病和186例乳腺癌中MUC2和MMP-9基因的表达，免疫印迹法观测MUC2和MMP-9蛋白表达情况。分析两者与乳腺癌临床病理特征之间的关系及两者的相关性。 结果 MUC2和MMP-9 mRNA在乳腺癌中的表达明显高于乳腺良性疾病组。乳腺良性疾病组织中MUC2和MMP-9 mRNA的表达率分别为2.9%（2/68）、13.2%（9/68），均显著低于癌组织39.8%（53/186）、73.1%（136/186）（P < 0.05）。类似于基因表达，MUC2和MMP-9蛋白在乳腺癌中的表达相对较高。MUC2和MMP-9基因在乳腺癌中的表达与病理类型、淋巴结转移相关（P < 0.05），MMP-9表达还与肿瘤大小和组织学分级有关（P < 0.05），两者均与患者年龄无关（P>0.05）。乳腺癌组织中MUC2和MMP-9 mRNA的表达呈负相关（r=-0.814，P < 0.001）。 结论 MUC2和MMP-9联合检测可作为诊断乳腺疾病的指标之一，为乳腺癌诊断和治疗提供参考。 Abstract:Objective To investigate the MUC2 and MMP-9 expression in breast carcinoma tissue and to analyze its relationships with clinical characteristics. Methods Reverse transcription PCR was applied to detect the MUC2 and MMP-9 mRNA expression in benign breast diseases and breast cancer. The expression levels of MUC2 and MMP-9 were measured by Western blot. The correlations of MUC2 and MMP-9 with clinical characteristics and the relationship between these two proteins were analyzed. Results The grayscale bands of the MUC2 and MMP-9 mRNAs in breast tumors were significantly higher than those in benign breast diseases. The expression levels of MUC2 mRNA in benign breast diseases and breast carcinoma were 2.9% (2/68) and 39.8% (53/186), respectively (P < 0.05), whereas those of MMP-9 mRNA were 13.2% (9/68) and 73.1% (136/186), respectively (P < 0.05). Moreover, the MUC2 and MMP-9 expression was significantly lower in benign diseases than in breast tumors. The MUC2 and MMP-9 mRNA expressions were correlated with the tumor type and lymph-node metastasis of breast carcinoma, respectively (P < 0.05). In addition, the MMP-9 mRNA expression was correlated with histological grade and tumor size. However, no significant associations were found between gene expression and patient age (P>0.05). The MMP-9 expression had a significantly inverse correlation with MUC2 (r= -0.814; P < 0.001). Conclusion The MUC2 and MMP-9 expression may be indicators of breast cancer. They may be suitable indicies for diagnosis and treatment of breast carcinoma. -
Key words:
- Breast cancer /
- MUC2 /
- MMP-9
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图 1 MUC2、MMP-9 mRNA在乳腺组织中的表达
M：Marker；1：乳腺浸润性癌组；2：乳腺原位癌组；3：乳腺良性疾病组；4：黏液腺癌
Figure 1. Expression of MUC2 and MMP-9 mRNA in breast tissues
图 2 MUC2、MMP-9蛋白在乳腺组织中的表达
M：Marker；1：乳腺浸润性癌组；2：乳腺原位癌组；3：乳腺良性疾病组；4：黏液腺癌
Figure 2. Expression of MUC2 and MMP-9 protein in breast tissue
图 3 乳腺癌组织中MUC2和MMP-9 mRNA表达的相关性
Figure 3. Correlation between MUC2 and MMP-9 mRNA expression in breast carcinoma tissue
表 1 MUC2、MMP-9 mRNA在乳腺癌和良性组织中的表达 例（%）
Table 1. Expressions MUC2 and MMP-9 mRNA in breast benign disease and breast carcinoma tissue
表 2 乳腺癌中MUC2和MMP-9 mRNA表达与临床病理特征的关系 例（%）
Table 2. Association of MUC2 and MMP-9 mRNA expression with clinical characteristics
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