王玉亮, 郭仁德, 张彧. 胰腺癌组织中转化生长因子β诱导蛋白表达及临床意义[J]. 中国肿瘤临床, 2012, 39(2): 77-79. DOI: 10.3969/j.issn.1000-8179.2012.02.005
引用本文: 王玉亮, 郭仁德, 张彧. 胰腺癌组织中转化生长因子β诱导蛋白表达及临床意义[J]. 中国肿瘤临床, 2012, 39(2): 77-79. DOI: 10.3969/j.issn.1000-8179.2012.02.005
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Yuliang WANG, Rende GUO, Yu ZHANG. Expression of Transforming Growth Factor-β-induced Protein in Pancreatic Cancer and Its Significance[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2012, 39(2): 77-79. DOI: 10.3969/j.issn.1000-8179.2012.02.005
Citation: Yuliang WANG, Rende GUO, Yu ZHANG. Expression of Transforming Growth Factor-β-induced Protein in Pancreatic Cancer and Its Significance[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2012, 39(2): 77-79. DOI: 10.3969/j.issn.1000-8179.2012.02.005
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胰腺癌组织中转化生长因子β诱导蛋白表达及临床意义

Expression of Transforming Growth Factor-β-induced Protein in Pancreatic Cancer and Its Significance

    摘要
  • 摘要:
      目的  探讨胰腺癌组织中转化生长因子β诱导蛋白(TGFBIp/βig-h3)mRNA和蛋白表达水平及其意义。
      方法  收集15例胰腺癌术后的患者癌组织、癌旁组织以及4例正常胰腺组织标本, 提取胰腺组织标本总RNA, 利用半定量逆转录聚合酶链反应(RT-PCR)检测胰腺组织标本TGFBIp mRNA水平; 应用Western blot检测TGFBIp蛋白表达水平。
      结果  胰腺癌组织TGFBIpmRNA及蛋白表达水平明显高于癌旁胰腺组织及正常胰腺组织(P < 0.01)。在不同分化程度的胰腺癌组织中, TGFBIp mRNA及蛋白表达水平差异无统计学意义(P>0.05);Ⅲ~Ⅳ期及有淋巴结转移胰腺癌组织中TGFBIp mRNA及蛋白表达水平明显高于Ⅰ~Ⅱ期及无淋巴结转移胰腺癌组织, 差异有统计学意义(P < 0.05)
      结论  TGFBIp高表达与胰腺癌的发生发展相关, 其可能的调控机制及生物学效应有待进一步研究。

     

    Abstract:
      Objective  To investigate expression levels and significance of transforming growth factor-β-induced protein (TGFBIp/βig-h3) mRNA and protein in pancreatic cancer tissue samples.
      Methods  Total RNA was extracted from 15 pancreatic cancer, adjacent non-neoplastic parenchymal, and four normal pancreatic tissues. TGFBIp mRNA levels were detected with semi-quantitative reverse transcriptase-polymerase chain reaction. TGFBIp expression levels were investigated with Western blot.
      Results  The TGFBIp mRNA and protein levels increased significantly in the pancreatic cancer tissue compared with those in adjacent non-neoplastic parenchymal and normal pancreatic tissue (P < 0.01). There was no statistically significant difference among the different TGFBlp expression levels in tumor tissue (P>0.05), whereas a statistically significant difference was found between TGFBIp and tumor stage or lymph node metastasis (P < 0.05).
      Conclusion  High TGFBIp expression is associated with pancreatic cancer. The regulatory mechanism of the overexpression of TGFBIp and its role in pancreatic cancer is worthy of further evaluation.

     

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