Abstract:
Objective The study aims to investigate the effects of the expression and proliferation of the actin depolymerizing factor (ADF) on the migration and invasion of human colon cancer SW480 cells using diallyl disulfide (DADS).
Methods The proliferation, migration, and invasion potentials of ADF were examined using MTT, scratch healing, and transwell membrane assays. The expression levels of destrin and cofilin 1 were detected using the reverse transcription-polymerase chain reaction (RT - PCR) and Western blot analysis of SW480 cells, respectively.
Results The MTT assay results showed that the proliferation of SW480 cells treated with different concentrations of DADS for 24, 48, 72, and 96 h was significantly inhibited (P < 0.05). This result indicatedsd that DADS could suppress the proliferation of SW480 cells in a time- and dose-dependent manner. The scratch healing assay obtained cell migration rates of 55.51 %, 34.72 %, 23.23 %, and 12.87 % for SW480 cells treated with 30, 40, 50, and 60 mg·L-1 of DADS, respectively, for 48 h. These values are significantly lower than the migration rates of the untreated cells (75.86 %) and the SW480 cells treated with 20 mg·L-1 of DADS (72.58 %) (P < 0.05). These results showed that DADS could inhibit the migration of SW480 cells in a dose-dependent manner. Moreover, the transwell invasion assay results showed that the number of cells permeating through the Matrigel significantly decreased to 34.67 %, 50.54 %, 57.12 %, and 64.59 % when the SW480 cells were treated with 20, 30, 40, 50, and 60 mg·L-1 of DADS for 24 h (P < 0.05), respectively. These results demonstrated that DADS can repress the invasion of SW480 cells in a dose-dependent manner. The RT-PCR and Western blot analysis results showed that the expression of destrin mRNA and protein was downregulated to 24.7 % and 60.1 % and 30.1 % and 58.9 % (P < 0.05), respectively. The expression of cofilin 1 mRNA and protein was not statistically different from those in the SW480 cells treated with 45 mg· L-1 of DADS (P > 0.05). However, the expression of p-cofilin 1 notably decreased to 18.9 %, 53.8 %, 62.1 %, and 78.2 % after 1, 15, 30, and 60 min, respectively (P < 0.05).
Conclusion DADS inhibited the proliferation, migration, and invasion of SW480 cells by down-regulating the expression of destrin and p-cofilin 1.