肺癌患者外周血调节性T细胞和IL-10的检测及其意义

翟晋芳; 杜凤兰; 绳晋雅; 郭志娟

doi:10.3969/j.issn.1000-8179.2012.06.007

肺癌患者外周血调节性T细胞和IL-10的检测及其意义

Detection of CD4+CD25+ T-Cells and IL-10 in Peripheral Blood of Lung Cancer Patients ant Its Significance

摘要

摘要:
目的探讨肺癌患者外周血调节性T细胞和IL-10检测的诊断意义及与临床特征相关性。
方法采用流式细胞仪检测60例肺癌患者外周血中CD4⁺CD25⁺T细胞的表达水平, 同时采用ELISA法检测IL-10的表达水平。并随机选30例健康体检者作为对照组。
结果肺癌患者外周血中CD4⁺CD25⁺T细胞和IL-10的表达水平明显高于健康对照组（P < 0. 05）; CD4⁺CD25⁺T细胞与IL-10的检测结果呈正相关; 肺癌患者外周血中CD4⁺CD25⁺T细胞和IL-10的表达水平与临床分期有关（P < 0. 05）, 与病理类型无关（P>0. 05）。
结论检测肺癌患者外周血中CD4⁺CD25⁺T细胞和IL-10的表达水平, 有助于肺癌患者病情的评估和治疗。

Abstract:
Objective This study discusses the relationship of the CD4⁺CD25⁺T-cells and IL-10 in the peripheral blood of lung cancer patients with clinicopathologic features.
Methods The amounts of CD4⁺CD25⁺T-cells in the peripheral blood samples from 60 patients were measured using flow cytometry, whereas the amounts of IL-10 were measured using enzyme-linked immunosorbent assay. Blood samples from 30 healthy volunteers were also collected and examined.
Results The amounts of CD4⁺CD25⁺T-cells and IL-10 in the peripheral blood samples from lung cancer patients were significantly higher than those from the healthy volunteers (P < 0.05). Moreover, the amounts of CD4⁺CD25⁺T-cells and IL-10 were positively correlated. Finally, the amounts of CD4⁺CD25⁺T-cells and IL-10 were correlated with the clinical stage of the disease (P < 0.05) but were not associated with the pathological classification (P > 0.05).
Conclusion Determining the amounts of CD4⁺CD25⁺ T-cells and IL-10 may help in the assessment and therapy of lung cancer patients.

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