抗MUC1单克隆抗体C595对人卵巢癌0VCAR-3细胞增殖和凋亡的研究

陈红敏; 张明川; 罗艳林; 王莉

doi:10.3969/j.issn.1000-8179.2012.07.001

抗MUC1单克隆抗体C595对人卵巢癌0VCAR-3细胞增殖和凋亡的研究

Effects of Anti-MUC1 Monoclonal Antibody C595 on the Proliferation and Apoptosis of Human Ovarian Cancer Cell Line OVCAR-3

摘要

摘要:
目的探讨抗MUCI单克隆抗体C595在体外抑制高表达MUC1的人卵巢癌OVCAR-3细胞增殖并诱导其凋亡的作用。
方法免疫荧光标记法和流式细胞术检测单克隆抗体C595标记的MUC1在OVCAR-3细胞表面的表达; MTT法、克隆形成试验检测处理后细胞生长抑制情况, TUNEL法检测细胞凋亡, EIISA检测细胞内细胞色素C和Caspase-3活性的变化。
结果单克隆抗体C595对人卵巢癌OVCAR-3细胞具有明显的增殖抑制作用, 且呈剂量-时间依赖性, TUNEL结果表明C595(IC₅₀)可以诱导细胞凋亡。ELLSA检测显示治疗后的细胞凋亡与释放细胞色素C和Caspase-3浓度的上升相关。
结论单克隆抗体C595可以在体外抑制人卵巢癌OvCAR-3细胞增殖并诱导其凋亡, 其机制可能与上调Caspase-3蛋白活性有关。

Abstract:
Objective To investigate the effect of anti-MUC 1 monoclonal antibody (Mab) C595 on the growth and apoptosis of human ovarian cancer cell line OVCAR-3.
Methods The expression of MUC 1 on OVCAR-3 cells was examined by immunofluorescence labeling and flow cytometry using MabC595. The cell proliferation was measured by MTT assay and colony formation assay. TUNEL method was used to detect apoptosis. Cytochrome c and Caspase-3 activity were measured by ELISA.
Results Mab C595 inhibited the growth of OVCAR-3 cells in a concentration-and time-dependent manner. TUNEL showed that Mab C595 induced apoptosis of OVCAR-3. ELISA analysis showed that cell death following treatment was associated with the release of cytochrome c and Caspase-3 concentrations.
Conclusion Mab C595 inhibits the proliferation and induces cell apoptosis in human ovarian cancer cell line OVCAR-3. The mechanisms of cell apoptosis induced by Mab C595 may be through upregulating Caspase-3 activity.

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