Abstract:
Objective The current work aims to investigate the effects of metadherin (MTDH) down-regulation on cell proliferation, adhesion, and migration of human breast cancer MDA-MB-453 cells.
Methods RNA interference was employed to reduce MTDH expression in human breast cancer MDA-MB-453 cells. Reverse transcription polymerase chain reaction (RT-PCR) and immunocytochemistry were applied to ascertain the down-regulation of MTDH. 3-(4, 5-Dimethylthiazol-2-yl) -2, 5-diphenyltetrazolium bromide assay, cell adhesion, and migration assay were performed to respectively assess the changes in cell proliferation, adhesion, and migration.
Results The transfection efficiency of MTDH-siRNA reached 90 %. Forty-eight hours after transfection, the expression of MTDH mRNA and protein was reduced by 45.8 % and 47.5 %, respectively, compared with the control group. The knockdown of MTDH inhibited cell proliferation, and the inhibition ratio was 41.5 % and 49.0 % at 24 h and 48 h, respectively. Cell adhesion was also inhibited. The adhesion rate at 30 min and 60 min was decreased by 42.0 % and 49.7 %, respectively, compared with the control. Moreover, MTDH down-regulation resulted in a decreased migration rate of 33.3 %.
Conclusion The reduced MTDH expression in MDA-MB-453 cells can inhibit proliferation, adhesion, and migration, which suggests that MTDH plays an important role in the malignant biological behaviors of breast cancer cells.