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摘要:
目的 探讨IQGAP1（IQ motif containing GTPase activating protein 1）在乳腺癌组织中的表达及意义。 方法 采用免疫组织化学链霉菌抗生物素蛋白-过氧化物酶（S-P法）染色法检测IQGAP1在乳腺癌组织及癌旁组织各110例中的蛋白表达水平。在正常乳腺上皮细胞系MCF-10A和乳腺癌细胞系MCF-7中开展IQGAP1表达调控实验。 结果 IQGAP1在乳腺癌组织中的表达高于癌旁正常乳腺组织（P=4.23×10-6）。IQGAP1的表达与浸润性导管癌的肿瘤大小（P=0.006）相关，在肿瘤>2 cm组明显高于肿瘤≤2 cm组。未发现与淋巴结转移（P=0.870），临床分期（P=0.278），ER状态（P=0.412）和PR状态（P=0.248）相关。IQGAP1高表达与乳腺癌术后无病生存时间（P=0.008）和总生存时间（P=0.029）相关。在乳腺癌细胞系MCF-7中，IQGAP1降低导致细胞增殖活性显著下降。 结论 IQGAP1可能在乳腺癌的发生发展过程中发挥重要作用。 Abstract:Objective To investigate the expression of IQGAP1 in breast cancer tissues and their tumor-adjacent normal tissues and the effect of IQGAP1 on the proliferation of breast cancer cells. Methods A total of 110 breast invasive ductal cancer (IDC) and its adjacent normal tissues specimens were screened for the expression of IQGAP1 with streptavidin-perosidase (S-P) immunohistochemistry. IQGAP1 gene expression was regulated in MCF-7 and MCF-10A cell line. Results The expression of IQGAP1 was higher in breast cancer tissues than in tumor-adjacent normal tissues (P = 4.23×10-6). The expression of IQGAP1 was correlated with tumor size, and was significantly higher in tumor > 2 cm than in tumor ≤ 2 cm (P = 0.006), but was not correlated with lymphnode metastasis (P = 0.870), clinical staging (P = 0.278), expression of estrogen receptor (ER) (P = 0.412) or progestogen receptor (PR) (P = 0.248) in IDC. IQGAP1 high expression was associated with poor progression free time (P = 0.008) and overall survival time (P = 0.029) among breast cancer patients. The proliferation rate of MCF-7 cells was significantly decreased after knocking down IQGAP1 expression. Conclusion IQGAP1 may play important roles in the development of breast cancer. -
Key words:
- IQGAP1 gene /
- Breast cancer /
- Tumor-adjacent normal tissue /
- Immunohistochemistry /
- Cell proliferation
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图 1 IQGAP1在乳腺癌组织中的表达（S-P×400）
A：（-）；B：（+）；C：（++）；D：（+++）
Figure 1. The expression of IQGAP1 in breast cancer tissues
图 2 IQGAP1高表达组和低表达组的生存曲线
A：无病生存曲线；B：总生存曲线
Figure 2. The survival curves of groups with high IQGAP1 expression or low expression
图 3 乳腺癌细胞系与正常乳腺上皮细胞系中IQGAP1基因沉默细胞增殖活性变化
Figure 3. The proliferation of MCF-7 and MCF-10A cells after knockdown of IQGAP1 expression
表 1 IQGAP1在乳腺癌及癌旁组织中的表达
Table 1. The expression of IQGAP1 in breast cancer and tumor-adjacent normal tissues
表 2 IQGAP1的表达与浸润性导管癌临床特点的关系
Table 2. Correlation of IQGAP1 expression with clinical characteristics of IDC
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