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摘要:
目的 检测结直肠癌组织中Bmi-1、PTEN和E-Cadherin的表达，探讨三者表达的相关性及意义。 方法 用Western blot检测Bmi-1在5对结直肠癌和癌旁活检组织中的表达，实时定量PCR法检测Bmi-1、PTEN和E-Cadherin mRNA在28对结直肠癌和癌旁活检组织的表达；用免疫组织化学法检测Bmi-1、PTEN和E-Cadherin在28例结直肠癌石蜡组织中的表达及其相关性。 结果 Bmi-1蛋白在5例结直肠癌活检组织中的表达明显高于癌旁组织；Bmi-1 mRNA在89.3%（25/28）的癌组织中的表达高于癌旁组织；PTEN和E-Cadherin mRNA在82.1%（23/28）的癌组织中的表达低于癌旁组织；统计学分析发现Bmi-1和PTEN及E-Cad? herin的mRNA表达水平呈负相关；28例结直肠石蜡组织中，Bmi-1、PTEN和E-Cadherin蛋白的阳性率分别为92.9%（26/28）、46.4%（13/28）和82.1%（23/28），统计学分析发现Bmi-1和PTEN及E-Cadherin蛋白表达水平呈负相关。 结论 本研究发现Bmi-1和PTEN及E-Cadherin之间呈负相关，从组织水平证实Bmi-1对PTEN及E-Cadherin的调控关系，为Bmi-1作为预测结直肠癌发生及转移的分子标志物及治疗新靶点提供了新的理论依据。 -
关键词:
- 结直肠癌 /
- Bmi-1 /
- PTEN /
- E-Cadherin
Abstract:Objective To determine the expression of f Bmi-1, PTEN, and E-cadherin in colorectal cancer, and analyze the correlation among Bmi-1, PTEN and E-cadherin. Methods Western blot analysis was performed to detect Bmi-1 expression in five colorectal cancer biopsies and matched adjacent noncancerous tissues. Real-time reverse transcription polymerase chain reaction (RT-PCR) was performed to determine the protein and mRNA levels of Bmi-1, PTEN, and E-cadherin in 28 colorectal cancer biopsies and matched adjacent non-cancerous tissues. The protein levels of Bmi-1, PTEN, and E-cadherin in 28 paraffin-embedded colorectal cancer tissues were detected via immunohistochemistry. Statistical analysis was performed to evaluate the association among Bmi-1, PTEN, and E-cadherin at both the mRNA level and the protein level. Results Bmi-1 was clearly upregulated in the 5 colorectal cancer biopsies and their matched adjacent non-cancerous tissues. Bmi-1 mRNA was upregulated in 89.3% (25/28) of colorectal cancer biopsies compared with the adjacent noncancerous tissues. PTEN and E-cadherin mRNA were downregulated in 82.1% (23/28) of colorectal cancer biopsies compared with adjacent noncancerous tissues. High Bmi-1 mRNA expression was significantly correlated with low levels of PTEN and E-cadherin mRNA. Immunohistochemistry and statistical analyses revealed that high Bmi-1 protein expression was significantly associated with low levels of PTEN and E-cadherin. Conclusion This study provided evidence of the negative regulation of PTEN and E-cadherin by Bmi-1 at the clinical tissue level. Bmi-1 is a biomarker for colorectal cancer progression and metastasis and may be used as a therapeutic target. -
Key words:
- Colorectal cancer /
- Bmi-1 /
- PTEN /
- E-Cadherin
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图 1 Western blot法检测Bmi-1蛋白在结直肠癌组织及癌旁组织中的表达
Figure 1. Western blot analyses of Bmi-1 protein in colorectal cancer biopsies and matched adjacent tissues
图 2 Real-time PCR法检测Bmi-1、PTEN和E-Cadherin mRNA的表达
Figure 2. Analyses of Bmi-1, PTEN, and E-cadherin mRNA in colorectal cancer biopsies and matched adjacent tissues via real-time PCR
图 3 Bmi-1、PTEN和E-Cadherin mRNA在癌组织和癌旁组织中的表达比较
Figure 3. Bmi-1, PTEN, and E-cadherin mRNA expression in colorectal cancer biopsies and matched adjacent tissues
图 4 Bmi-1、PTEN和E-Cadherin蛋白在分化程度较低的结直肠癌组织中的表达（×400）
Figure 4. Bmi-1, PTEN, and E-cadherin protein expression in lowly differentiated colorectal cancer tissues
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