Relationship of Oct-4 and Sox-2 Expression in Colon Carcinoma with Recurrence and Metastasis
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摘要:
目的 探讨结肠癌组织中Oct-4、Sox-2表达情况及其对术后复发转移的预测作用。 方法 采用免疫组织化学方法检测手术切除的80例结肠癌术后标本中Oct-4、Sox-2表达情况，并且对两指标的表达同肿瘤分化程度、分期和术后复发转移关系进行分析。采用RT-PCR检测20例冰冻肿瘤组织及癌旁组织中Sox-2、Oct-4表达情况。 结果 80例患者中35例出现复发转移，Sox-2、Oct-4在转移组中表达率分别为48.57%（17/35）、51.43%（18/35），在非转移组中表达率分别为17.78%（8/45）、13.33%（6/ 45），差异有统计学意义（P=0.001）。原发病灶分化程度、T分期和N分期同Oct-4和Sox-2表达无显著性差异。均为阳性表达者其转移发生率高，生存分析显示不同表达状态转移出现时间差异有统计学意义（P=0.001）。RT-PCR分析显示20例肿瘤组织中Sox-2、Oct-4阳性表达率分别为40%（8/20）、45%（9/20），明显高于癌旁正常组织5%（1/20）。 结论 肿瘤组织中Sox-2、Oct-4表达同结肠癌术后转移相关，两者联合检测有助于评估结肠癌术后复发转移的可能。 Abstract:Objective The current work aims to investigate the expression of Sox-2 and Oct-4 in colon carcinoma and its relationship with relapse and metastasis. Methods The expression of Oct-4 and Sox-2 was detected in 80 cases of colon cancer tissue by immunohistochemical assay. The correlation of the Oct-4 and Sox-2 expression with histological differentiation, T stage, N stage, and metastasis was analyzed. The level of Oct-4 and Sox-2 expression in the cancer and normal paraneoplastic tissues was evaluated using RT-PCR. Results Metastasis was observed in 35 of all the 80 cases studied. The positive rate of Sox-2 and Oct-4 in the metastasis group was 48.57% (17/35) and 51.43% (18/35), respectively, whereas it was 17.78% (8/45) and 13.33% (6/45) in the non-metastasis group. Significant differences were observed between the two groups (P = 0.001). The expression of Oct-4 and Sox-2 was not associated with differentiation, T stage, or N stage of the tumor. The group with positive expression of Oct-4 and Sox-2 showed high metastatic rate. The survival analysis showed that the time of metastasis was significantly different in the groups with various expressions (P = 0.001). The positive expression rate of Sox-2 and Oct-4 detected by RT-PCR in colonic carcinoma tissues was 40% (8/20) and 45% (9/20) respectively, higher than that in normal tissues. Conclusion The expression of Sox-2 and Oct-4 in tumor tissue can predict postoperative relapse and metastasis of colon carcinoma. -
Key words:
- Colon carcinoma /
- Oct-4 /
- Sox-2 /
- Metastasis /
- Recurrence
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图 1 Oct-4、Sox-2在结肠癌组织中表达（S-P×400）
A：Oct-4阳性；B：Sox-2阳性
Figure 1. Expression of Oct-4 and Sox-2 in colon cancer
图 2 RT-PCR检测Oct-4、Sox-2在结肠癌组织及癌旁组织中的表达
T：肿瘤组织；N：癌旁组织；B：空白对照
Figure 2. Expression of Oct-4 and Sox-2 in colon cancer and normal paraneoplastic tissues
图 3 Oct-4、Sox-2阳性表达和阴性表达患者转移出现时间比较（以转移为截点）
Figure 3. Comparison of metastasis between patients with Oct-4 and Sox-2 expression and those without Oct-4 and Sox-2 expression(P=0.001)(metastasis was censored)
表 1 引物序列
Table 1. Primer sequences
表 2 组织中Sox-2、Oct-4表达同临床特征间关系
Table 2. Relationship of Sox-2 and Oct-4 expression with clinical characteristics
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