周杰, 覃佳强, 金先庆, 宿玉玺, 谢艳, 南国新, 王忠良, 刘星, 张德文. 胰岛素样生长因子结合蛋白5对人骨肉瘤细胞增殖与侵袭的影响[J]. 中国肿瘤临床, 2012, 39(10): 626-629. DOI: 10.3969/j.issn.1000-8179.2012.10.002
引用本文: 周杰, 覃佳强, 金先庆, 宿玉玺, 谢艳, 南国新, 王忠良, 刘星, 张德文. 胰岛素样生长因子结合蛋白5对人骨肉瘤细胞增殖与侵袭的影响[J]. 中国肿瘤临床, 2012, 39(10): 626-629. DOI: 10.3969/j.issn.1000-8179.2012.10.002
shu
Jie ZHOU, Jia-qiang TAN, Xian-qing JIN, Yu-xi SU, Yan XIE, Guo-xin NAN, Zhong-liang WANG, Xing LIU, De-wen ZHANG. Effects of Insulin-like Growth Factor Binding Protein 5 on the Proliferation and Invasion of Osteosarcoma Cells[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2012, 39(10): 626-629. DOI: 10.3969/j.issn.1000-8179.2012.10.002
Citation: Jie ZHOU, Jia-qiang TAN, Xian-qing JIN, Yu-xi SU, Yan XIE, Guo-xin NAN, Zhong-liang WANG, Xing LIU, De-wen ZHANG. Effects of Insulin-like Growth Factor Binding Protein 5 on the Proliferation and Invasion of Osteosarcoma Cells[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2012, 39(10): 626-629. DOI: 10.3969/j.issn.1000-8179.2012.10.002
shu

胰岛素样生长因子结合蛋白5对人骨肉瘤细胞增殖与侵袭的影响

Effects of Insulin-like Growth Factor Binding Protein 5 on the Proliferation and Invasion of Osteosarcoma Cells

    摘要
  • 摘要:
      目的  探讨胰岛素样生长因子结合蛋白5(insulin-like growth factor binding protein 5, IGFBP5)对人骨肉瘤细胞株143B增殖及侵袭力的影响。
      方法  用IGFBP5过表达重组腺病毒(Ad-IGFBP5)、IGFBP5 RNA干扰重组腺病毒(Ad-siIGFBP5)和空载红色荧光蛋白重组腺病毒(Ad-RFP)感染人骨肉瘤细胞株143B。通过细胞增殖、划痕愈合、细胞迁移室(Transwell)侵袭等多种实验方法, 研究IGFBP5对人骨肉瘤细胞株生长、迁移与侵袭的影响。
      结果  Ad-IGFBP5组感染后143B细胞生长速度低于Ad-RFP组(P < 0.05);Ad-IGFBP5组细胞在划痕后16h即完全愈合, 划痕愈合速度显著低于Ad-RFP组(P < 0.05);24 h后Ad-IGFBP5组侵袭至Matrigel胶上的平均细胞数量较Ad-RFP组减少(P < 0.05)。而Ad-siIGFBP5感染后, 143B细胞表现为生长率升高, 划痕愈合速度加快及侵袭力增强。
      结论  IGFBP5能抑制骨肉瘤细胞增殖、迁移、侵袭。提示IGFBP5在抑制骨肉瘤发生及转移中可能起到重要作用。

     

    Abstract:
      Objectives  This work aims to investigate the effect of insulin-like growth factor binding protein 5(IGFBP5) on the proliferation and invasion of human osteosarcoma cells(143B).
      Methods  The effects of IGFBP5 on the growth, migration, and invasion of osteosarcoma 143B cells were studied.The 143B cell lines were infected with IGFBP5-overexpressing recombinant adenovirus(Ad -IGFBP5) and small interfering RNA-mediated knockdown of IGFBP5 recombinant adenovirus(Ad - silGFBP5).Cell proliferation, cell wound healing assay, and Transwell invasion assay were performed to determine the effect of IGFBP5 on the osteosarcoma cells.
      Results  Cell proliferation assay and functional analysis revealed that the osteosarcoma cells infected with Ad-IGFBP5 had significantly lower cell growth rates, wound closure rates, and invasiveness compared with the cells in the control group(P < 0.05).Meanwhile, after infection with Ad-siIGFBP5, the cells exhibited the opposite biological effects.
      Conclusion  IGFBP5 may inhibit the tumorigenesis and metastasis of osteosarcoma.

     

    • HTML全文
    • 参考文献(13)
    • 相关文章
    • 施引文献
  • 资源附件(0)

/

返回文章
返回