肝细胞肝癌组织中Bcl-2核表达及MMP-2和MMP-9表达与术后复发转移及预后的关系

孙涛; 孙保存; 赵秀兰; 赵楠; 古强; 车娜; 董学易; 池嘉栋

doi:10.3969/j.issn.1000-8179.2012.11.004

肝细胞肝癌组织中Bcl-2核表达及MMP-2和MMP-9表达与术后复发转移及预后的关系

doi: 10.3969/j.issn.1000-8179.2012.11.004

孙涛^①,
孙保存^{①, ②, ,},
赵秀兰^①,
赵楠^①,
古强^①,
车娜^①,
董学易^①,
池嘉栋^①

①.
天津医科大学病理教研室（天津市300070）
②.
天津医科大学附属肿瘤医院病理科天津市肿瘤防治重点实验室

基金项目:

国家自然科学基金重点项目 30830049

面上项目 30830049

天津市科技支撑项目 09ZCZDSF04400

详细信息

通讯作者:
孙保存 sunbaocun@yahoo.com.cn

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出版历程
- 收稿日期: 2012-04-16
- 修回日期: 2012-05-19

Relationship of Bcl-2 Expression with MMP2 and MMP9 Expression in Hepatocellular Carcinoma, and Their Correlation with Recurrence, Metastasis, and Prognosis

Tao SUN^①,
Baocun SUN^{①, ②
, ,},
Xiulan ZHAO^①,
Nan ZHAO^①,
Qiang GU^①,
Na CHE^①,
Xueyi DONG^①,
Jiadong CHI^①

①.
Department of Pathology, Tianjin Medical University, Tianjin 300070, China
②.
Department of Pathology, Cancer Institue and Hospital of Tianjin Medical University, Key Laboratory of Cancer Prevention and Treatment of Tianjin City, Tianjin 300060, China

Funds:

the Key Program of the National Natural Science Foundation of China 30830049

the National Natural Science Foundation of China 30830049

the Cooperation Project of China-Sweden 09ZCZDSF04400

More Information

Corresponding author: Baocun SUN; E-mail: sunbaocun@yahoo.com.cn

摘要

摘要: 目的探讨抗凋亡蛋白Bcl-2、基质金属蛋白酶-2(MMP-2)和MMP-9在肝细胞肝癌中表达的关联性, 及其与复发、转移和预后的关系。方法以石蜡包埋组织切片, 免疫组织化学SP法染色检测97例肝细胞肝癌, 分别在复发与转移组49例和非复发与转移组48例中分析Bc1-2核表达和MMP-2、MMP-9表达的情况。结果复发与转移组Bcl-2核表达高于无复发与转移组(x²=7.912, P=0.005), 复发转移组MMP-9表达高于无复发转移组(x²=6.545, P=0.011), Bcl-2核表达阳性细胞率与MMP-9阳性细胞率存在相关性, 而与MMP-2无关Bcl-2核表达组患者生存时间较短, 差异具有统计学意义。结论 Bcl-2核表达阳性患者更易出现复发与转移, 且与MMP-9过度表达存在关联性, 表明Bcl-2入核可能对肿瘤转移的相关生物学功能具有调节作用, 可作为评价HCC复发与转移、不良生存预后的候选临床标志。
- Bcl-2 /
- MMP-2 /
- MMP-9 /
- 复发与转移
Abstract: Objective To determine the correlation of the expression of the anti-apoptotic protein Bcl-2 with the expression of matrix metalloproteinases MMP-2 and MMP-9 in human hepatocellular carcinoma, and their relationship with recurrence, metastasis, and prognosis. Methods Paraffin-embedded tissue sections of 97 hepatocellular carcinoma cases were subjected to SP immunohistochemical staining.The nuclear expression of Bcl-2 as well as the expression of MMP-2 and MMP-9 were detected.A total of 49 cases had recurrence and metastasis, and the remaining 48 did not. Results The nuclear expression of Bcl-2 was higher in cases with recurrence and/or metastasis(t = -2.488, P = 0.015).Similarly, MMP-2 and MMP-9 expression were higher in cases with recurrence and/or metastasis(Z = -2.623, P = 0.009;Z = -2.028, P = 0.043).The nuclear expression of Bcl-2 was correlated with the expression of MMP-9, but not with that of MMP-2.Patients with positive nuclear expression of Bcl-2 had shorter survival time, and the difference was significant. Conclusion The nuclear expression of Bcl-2 was significantly correlated with metastasis and MMP-9 expression. Bcl-2 relocation into the nucleus may regulate metastasis-related proteins, which can be used as a clinical marker for evaluating recurrence, metastasis, and prognosis.
- Bcl-2 /
- MMP-2 /
- MMP-9 /
- Recurrence and metastasis

HTML全文

图 1 MMP-2、MMP-9阳性细胞率与BCl-2核阳性细胞率间的相关性分析

Figure 1. Correlation of the positive rates of MMP-2 and MMP-9 with Bcl-2

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图 2 Bcl-2浆表达和核表达与生存时间的关系

Figure 2. Relationship between Bcl-2 expression and survival time

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表 1 肝癌Bcl-2、MMP-2和MMP-9阳性细胞率与复发转移的关系例

Table 1. Relationships of the positive rates of Bcl-2, MMP-2, and MMP-9 with metastasis

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