IFT20蛋白在不同肿瘤组织中表达的研究

姜容; 刘贵勇; 陈鹏; 汪长东

doi:10.3969/j.issn.1000-8179.2012.12.007

IFT20蛋白在不同肿瘤组织中表达的研究

doi: 10.3969/j.issn.1000-8179.2012.12.007

姜容^①,
刘贵勇^②,
陈鹏^③,
汪长东^④, ,

①.
重庆医科大学干细胞与组织工程研究室（重庆市400016）
②.
重庆市巴南区人民医院手足外科
③.
广西大学农学院
④.
重庆医科大学医学分子与肿瘤研究中心, 生物化学与分子生物学教研室

基金项目:

国家自然科学基金项目 30900912

国家自然科学基金项目 31060199

详细信息

通讯作者:
汪长东 wangchangdonghust@yahoo.cn

计量
- 文章访问数: 50
- HTML全文浏览量: 1
- PDF下载量: 0
- 被引次数: 0
出版历程
- 收稿日期: 2011-11-05
- 修回日期: 2012-03-25

Expression of Intraflagellar Transport 20 Protein in Tumor Tissues

Rong JIANG^①,
Guiyong LIU^②,
Peng CHEN^③,
Changdong WANG^{④
, ,}

①.
Department of Stem Cell Tissue Engineering, Chongqing Medical University, Chongqing 400016, China
②.
Department of Surgery of Hand and Foot, The People's Hospital of Banan District, Chongqing 400320, China
③.
Agriculture College of Guangxi University, Guangxi University, Nanning 530005, China
④.
Department of Biochemistry and Molecular Biology, Chongqing Medical University, Chongqing 400016, China

Funds:

the National Natural Science Foundation of China 30900912

the National Natural Science Foundation of China 31060199

More Information

Corresponding author: Changdong WANG; Email: wangchangdonghust@yahoo.cn

摘要

摘要: 目的探讨IFT20（Intraflagellar Transport，IFT）蛋白在人卵巢癌、肺癌、胰腺癌、小肠癌、前列腺癌、骨癌、胃癌和乳腺癌癌组织和癌旁组织的表达分布情况，为人类肿瘤的诊断和治疗寻找新的靶点。方法采用免疫组织化学方法，研究IFT20蛋白在人的八种癌组织和癌旁组织的表达。结果结果发现，IFT20蛋白在卵巢癌组织中大量表达，在卵巢癌癌旁组织低量表达。IFT20蛋白在小肠癌，胃癌和乳腺癌组织中中量表达，在小肠癌，胃癌和乳腺癌癌旁组织中低量表达。IFT20蛋白在胰腺癌，骨癌，肺癌癌组织中低量表达，在胰腺癌、骨癌、肺癌癌旁组织中几乎不表达。IFT20蛋白在前列腺癌组织和癌旁组织中均未见表达。结论实验结果证实，IFT20蛋白可能经过多种途径直接或间接调节卵巢癌、肺癌、胰腺癌、小肠癌、骨癌、胃癌和乳腺癌的发生，发展过程，并且IFT20可能是卵巢癌、肺癌、胰腺癌、小肠癌、骨癌、胃癌和乳腺癌肿瘤药物治疗的潜在靶点。
- 细胞原纤毛 /
- 免疫组织化学 /
- IFT20 /
- 肿瘤
Abstract: Objective To find a new target for the diagnosis and treatment of human cancers by analyzing the expression of intraflagellar transport (IFT) 20 protein in human ovarian, lung, pancreatic, intestinal, prostate, bone, gastric, and breast cancers. Methods IFT20 protein expression in 8 human cancer tissues was detected by immunohistochemistry. Results IFT20 protein was highly expressed in ovarian cancer tissue, and only lowly expressed in surrounding tissues. IFT20 was expressed moderately in intestinal, gastric, and breast cancer tissues, but lowly in surrounding tissues. IFT20 protein was expressed slightly in bone, pancreatic, and lung cancer tissues. There was no significant IFT20 expression of protein in prostate cancer and surrounding tissues. Conclusion IFT20 can directly or indirectly regulate the initiation and development of ovarian, intestinal, gastric, breast, bone, pancreatic, and lung cancers. Therefore, IFT20 is a potential therapeutic target for cancer treatment.
- Primary cilia /
- Immunohistochemistry method /
- IFT20 /
- Tumor

HTML全文

图 1 8种肿瘤组织不同程度的IFT20蛋白表达（×200）

a1：卵巢癌癌旁组织；a2：卵巢癌组织；b1：小肠癌癌旁组织；b2：小肠癌组织；c1：胃癌癌旁组织；c2：胃癌组织；d1：乳腺癌癌旁组织；d2：乳腺癌组织；e1：胰腺癌癌旁组织；e2：胰腺癌组织；f1：肺癌癌旁组织；f2：肺癌组织；g1：骨癌癌旁组织；g2：骨癌组织；h1：前列腺癌癌旁组织；h2：前列腺癌组织

Figure 1. IFT20 protein expression in tissues of eight cancers

下载: 全尺寸图片幻灯片

参考文献(10)

[1]	Jonassen JA, San Agustin J, Follit JA, et al. Deletion of IFT20 in the mouse kidney causes misorientation of the mitotic spindle and cystic kidney disease[J]. J Cell Biol, 2008, 183(3): 377-384. doi: 10.1083/jcb.200808137
[2]	Evans RJ, Schwarz N, Nagel-Wolfrum K, et al. The retinitis pigmentosa protein RP2 links pericentriolar vesicle transport between the Golgi and the primary cilium[J]. Hum Mol Genet, 2010, 19(7): 1358-1367. doi: 10.1093/hmg/ddq012
[3]	Clement CA, Kristensen SG, Møllgård K, et al. The primary cilium coordinates early cardiogenesis and hedgehog signaling in cardiomyocyte differentiation[J]. J Cell Sci. 2009, 122(Pt 17): 3070-3082.
[4]	Sironen A, Hansen J, Thomsen B, et al. Expression of SPEF2 During Mouse Spermatogenesis and Identification of IFT20 as an Interacting Protein[J]. Biol Reprod. 2010, 82(3): 580-590. doi: 10.1095/biolreprod.108.074971
[5]	Smits P, Bolton AD, Funari V, et al. Lethal Skeletal Dysplasia in Mice and Humans Lacking the Golgin GMAP-210[J]. N Engl J Med. 2010, 362(3): 206-216. doi: 10.1056/NEJMoa0900158
[6]	Omori Y, Zhao C, Saras A, et al. Elipsa is an early determinant of ciliogenesis that links the IFT particle to membrane-associated small GTPase Rab8[J]. Nat Cell Biol, 2008, 10(4): 437-444. doi: 10.1038/ncb1706
[7]	Rubin LL, de Sauvage FJ. Targeting the Hedgehog pathway in cancer [J]. Nat Rev Drug Discov, 2006, 5(12): 1026-1033. doi: 10.1038/nrd2086
[8]	Hanahan D, Weinberg RA. Hallmarks of Cancer: The Next Generation [J]. Cell, 2011, 144(5): 646-674. doi: 10.1016/j.cell.2011.02.013
[9]	Keady BT, Le YZ, Pazour GJ. IFT20 is required for opsin trafficking and photoreceptor outer segment development[J]. Mol Biol Cell, 2011, 22(7): 921-930. doi: 10.1091/mbc.e10-09-0792
[10]	Follit JA, San Agustin1 JT, Xu F, et al. The golgin GMAP210/TRIP11 anchors IFT20 to the colgi complex[J]. PLoS Genet, 2008, 4(12): e1000315. doi: 10.1371/journal.pgen.1000315