Abstract:
Objective To clarify whether miR-146b-5p can modulate the expression of MMP-16 and to observe its effects on migration, invasion, proliferation, and apoptosis of the glioblastoma cell line.
Methods TJ905 cells were transfected with the expression plasmid of the nonsense scrambled sequence (Group 1) or that of miR-146b-5p (Group 2). The expression levels of miR-146b-5p, MMP 16 mRNA, and MMP 16 protein were measured by quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot assay. Migratory and invasive abilities and cell cycle distribution and apoptosis in the two groups were assessed by in vitro migration assays and by invasion and flow cytometry.
Results Compared with Group 1, the expression levels ofMMP16 mRNA and MMP16 protein significantly decreased in Group 2. MMP16 mRNA and MMP16 protein expression levels positively correlated with each other, whereas they negatively correlated with the expression level of miR-146b-5p. The number of migrated and invaded cells was significantly lower in Group 2 than in Group 1, which was positively correlated with the expression level of MMP 16. The apoptotic level was significantly higher in Group 2 than in Group 1 and was positively correlated with the miR-146b-5p expression level in Group 2. However, the two groups shared a similar pattern on cell cycle distribution.
Conclusion MiR-146b-5p is a tumor-suppressive mRNA against glioma. Exogenous miR-146b-5p can effectively promote apoptosis of the glioma cells and can inhibit invasive and migratory abilities by down-regulating the expression level of MMP16. Our results suggest a potential value of miR-146b-5p in the gene therapy of malignant gliomas.
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